Abstract:Objective To study scleral fibroblast proliferation and type I collagen compound in human fetal scleral fibroblasts (HFSFs) by applying the soluble guanylyl cyclase (sGC) antagonist (NS-2028). Methods Experimental study. HFSFs were divided into two groups. The experimental group was treated with NS-2028; the control group was not treated. The experimental concentrations were 0.1, 1.0, 10.0, 100.0 μmol/L. After NS-2028 stimulation, the changes in the HFSFs of the control group and experimental groups were analyzed with CCK8, realtime fluorescence quantitative PCR (RTFQ PCR) and immunofluorescence (IF). Results ①The OD value of HFSFs in the control group was 0.82±0.04. The OD values of the four NS-2028 treated groups were 0.81±0.05, 0.83±0.05, 0.85±0.05 and 0.81±0.05, there were no significant differences from the control group (F=0.620, P>0.05). ②The relative transcript levels of COL1A1 mRNA in the the four NS-2028 treated groups were 1.06±0.75, 1.19±0.68, 1.40±0.18, 1.88±0.24, there were significant differences compared to the control group(F=32.138, P<0.05). ③The relative expression level of COL1A1 in HFSFs increased in the four NS-2028 treated groups compared to the control group. Conclusion NS-2028 plays a significant role in promoting the composition of COL1A1. There may be no significant difference in the proliferation of HFSFs. However, COL1A1 may directly affect HFSFs, delaying the development of myopia.
李创,吕勇,赵宏. 可溶性鸟苷酸环化酶抑制剂对体外培养人胚胎眼巩膜成纤维细胞胶原合成的影响[J]. 中华眼视光学与视觉科学杂志, 2016, 18(7): 415-419.
Li Chuang, Lyu Yong, Zhao Hong. The regulation of human fetal scleral fibroblasts using soluble guanylyl cyclase antagonist in vitro. Chinese Journal of Optometry Ophthalmology and Visual science, 2016, 18(7): 415-419. DOI: 10.3760/cma.j.issn.1674-845X.2016.07.007
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