Objective To observe the effect of Puerarin on human fetal scleral fibroblasts′（HFSFs） proliferation and expression of collagen type I （COL1A1） following exposure to extremely low frequency electromagnetic field （ELF-EMFs）. Methods Experiment study. HFSFs were cultured and divided into control groups and radiation groups， which were exposed to 0.2 mT ELF-EMFs. The radiation groups were divided into groups of different puerarin concentrations （0.0， 0.1， 1.0， 10.0 µmol/L）. The proliferation of HFSFs under different exposure time （0， 12， 24， 48 hours） and under different final puerarin concentrations were detected by MTT skills. The expressions of COL1A1 mRNA and protein of all these groups were detected by real-time PCR and Western-Blot. Single factor analysis of variance was first used to determine whether there was difference among these groups， LSD-t was then used to figure out which groups had significant difference. And the difference between two groups are analysed by independent sample t test. Results There was significant difference between different radiating time in radiations groups （F=4.560， P<0.05）， and the proliferation of HFSFs （A490） of radiation groups was inhibited after exposed to 0.2 mT ELF-EMFs for 24 hours （t=5.000， P<0.01） with shrinking protein expression of COL1A1 （t=7.956， P<0.01） and decreasing mRNA expression （t=17.364， P<0.01）. However， Puerarin （1.0 µmol/L） enhanced HFSFs′ proliferation activity （P<0.01） and 0.1 µmol/L increased the protein expression of COL1A1 （P<0.05） and its mRNA expression （P<0.05）. The effects were more obvious under higher-concentration of puerarin（1.0， 10.0 µmol/L）. Conclusion ELF-EMFs inhibited the proliferation of HFSFs and expression of COL1A1， but puerarin can reverse these changes， suggesting that puerarin play a postive role in sclera remodeling.