Abstract： Corneal collagen cross-linking (CXL) can be used to treat progressive keratoconus, as well as infectious keratitis, keratolysis and corneal stroma edema. At present, riboflavin ultraviolet cross-linking is the commonly used clinical CXL scheme. The concentration and distribution of riboflavin in the cornea is one of the important factors affecting its efficacy and safety. Its main detection methods include fluorescence microscopy imaging, high performance liquid chromatography, spectrophotometry and noninvasive realtime detection of riboflavin concentration, observation of anterior segment slit lamp and imaging mass spectrometry by matrix-assisted laser desorption/ionization, etc. Different detection methods may lead to different results. This paper summarizes riboflavin concentration and its detection methods in current research.
严梦迪 黄锦海 王勤美 高蓉蓉. 角膜胶原交联中核黄素浓度检测方法的研究进展[J]. 中华眼视光学与视觉科学杂志, 2021, 23(8): 636-640.
Mengdi Yan, Jinhai Huang, Qinmei Wang, Rongrong Gao. Progress on Research of the Concentration Detection Method of Riboflavin in Corneal Collagen Cross-Linking. Chinese Journal of Optometry Ophthalmology and Visual science, 2021, 23(8): 636-640. DOI: 10.3760/cma.j.cn115909-20200316-00093
Ting DSJ, Henein C, Said DG, et al. Effectiveness of adjuvant photoactivatedchromophore corneal collagen crosslinking versus standard antimicrobial treatment for infectious keratitis: A systematic review protocol. JBI Database System Rev Implement Rep, 2020, 18(1): 194-199. DOI: 10.11124/ JBISRIR-D-19-00148.
[2]
Ting DSJ, Henein C, Said DG, et al. Photoactivatedchromophore for infectious keratitis-corneal cross-linking (PACK-CXL): A systematic review and meta-analysis. Ocul Surf, 2019, 17(4): 624-634. DOI: 10.1016/j.jtos.2019.08.006.
[3]
Cristian C, Marco CDV, Arturo K, et al. Accelerated collagen cross-linking in the management of advanced Acanthamoeba keratitis. Arq Bras Oftalmol, 2019, 82(2): 103-106. DOI: 10.5935/0004-2749.20190018.
Rubinfeld RS, Caruso C, Ostacolo C. Corneal Cross-Linking: The Science Beyond the Myths and Misconceptions. Cornea, 2019, 38(6): 780-790. DOI: 10.1097/ICO.0000000000001912.
[6]
N. K, VV. K. Delivery of riboflavin-5'-monophosphate into the cornea: can liposomes provide any enhancement effects?. J Pharm Sci, 2017, 106(10): 3041-3049. DOI: 10.1016/ j.xphs.2017.05.022.
[7]
Lombardo G, Villari V, Micali NL, et al. Non-invasive optical method for real-time assessment of intracorneal riboflavin concentration and efficacy of corneal cross-linking. J Biophotonics, 2018, 11(7): e201800028. DOI: 10.1002/ jbio.201800028.
[8]
Kamaev P, Friedman MD, Sherr E, et al. Photochemical kinetics of corneal cross-linking with riboflavin. Invest Ophthalmol Vis Sci, 2012, 53(4): 2360-2367. DOI: 10.1167/iovs.11-9385.
[9]
O'Brart NAL, O'Brart DPS, Aldahlawi NH, et al. An investigation of the effects of riboflavin concentration on the efficacy of corneal cross-linking using an enzymatic resistance model in porcine corneas. Invest Ophthalmol Vis Sci, 2018, 59(2): 1058-1065. DOI: 10.1167/iovs.17-22994.
[10]
Iseli HP, Popp M, Seiler T, et al. Laboratory measurement of the absorption coefficient of riboflavin for ultraviolet light (365 nm). J Refract Surg, 2011, 27(3): 195-201. DOI: 10.3928/1081597X- 20100604-01.
[11]
Spoerl E, Mrochen M, Sliney D, et al. Safety of UVA-riboflavin cross-linking of the cornea. Cornea, 2007, 26(4): 385-389. DOI: 10.1097/ICO.0b013e3180334f78.
[12]
Lin L, Wang Y, Xiao Y, et al. Ratiometric fluorescence detection of riboflavin based on fluorescence resonance energy transfer from nitrogen and phosphorus co-doped carbon dots to riboflavin. Anal Bioanal Chem, 2019, 411(13): 2803-2808. DOI: 10.1007/s00216-019-01725-1.
[13]
Gore DM, O'Brart D, French P, et al. Transepithelial riboflavin absorption in an ex vivo rabbit corneal model. Invest Ophthalmol Vis Sci, 2015, 56(8): 5006-5011. DOI: 10.1167/ iovs.15-16903.
[14]
Wang M, Zhang F, Zhao X, et al. Riboflavin concentration analysis in rabbit corneas before and after corneal collagen cross-linking using confocal laser scanning microscopy. AsiaPac J Ophthalmol (Phila), 2014, 3(6): 388-394. DOI: 10.1097/ APO.0000000000000100.
[15]
Gore DM, O'Brart DP, French P, et al. A comparison of different corneal iontophoresis protocols for promoting transepithelial riboflavin penetration. Invest Ophthalmol Vis Sci, 2015, 56(13): 7908-7914. DOI: 10.1167/iovs.15-17569.
[16]
Lanzini M, Curcio C, Spoerl E, et al. Confocal microscopy evaluation of stromal fluorescence intensity after standard and accelerated iontophoresis-assisted corneal cross-linking. Int Ophthalmol, 2017, 37(1): 235-243. DOI: 10.1007/s10792-016- 0266-8.
[17]
Sondergaard AP, Hjortdal J, Breitenbach T, et al. Corneal distribution of riboflavin prior to collagen cross-linking. Curr Eye Res, 2010, 35(2): 116-121. DOI: 10.3109/02713680903431847.
[18]
Laggner M, Pollreisz A, Schmidinger G, et al. Correlation between multimodal microscopy, tissue morphology, and enzymatic resistance in riboflavin-UVA cross-linked human corneas. Invest Ophthalmol Vis Sci, 2015, 56(6): 3584-3592. DOI: 10.1167/iovs.15-16508.
[19]
Seiler TG, Batista A, Frueh BE, et al. Riboflavin concentrations at the endothelium during corneal cross-linking in humans. Invest Ophthalmol Vis Sci, 2019, 60(6): 2140-2145. DOI: 10.1167/iovs.19-26686.
[20]
Wollensak G, Sporl E, Reber F, et al. Corneal endothelial cytotoxicity of riboflavin/UVA treatment in vitro. Ophthalmic Res, 2003, 35(6): 324-328. DOI: 10.1159/000074071.
[21]
Kampik D, Ralla B, Keller S, et al. Influence of corneal collagen crosslinking with riboflavin and ultraviolet-a irradiation on excimer laser surgery. Invest Ophthalmol Vis Sci, 2010, 51(8): 3929-3934. DOI: 10.1167/iovs.09-4524.
[22]
Gore DM, Margineanu A, French P, et al. Two-photon fluorescence microscopy of corneal riboflavin absorption. Invest Ophthalmol Vis Sci, 2014, 55(4): 2476-2481. DOI: 10.1167/iovs.14-13975.
[23]
Zhang Y, Sukthankar P, Tomich JM, et al. Effect of the synthetic NC-1059 peptide on diffusion of riboflavin across an intact corneal epithelium. Invest Ophthalmol Vis Sci, 2012, 53(6): 2620-2629. DOI: 10.1167/iovs.12-9537.
[24]
Arboleda A, Kowalczuk L, Savoldelli M, et al. Evaluating in vivo delivery of riboflavin with coulomb-controlled iontophoresis for corneal collagen cross-linking: a pilot study. Invest Ophthalmol Vis Sci, 2014, 55(4): 2731-2738. DOI: 10.1167/iovs.14-13931.
[25]
Baiocchi S, Mazzotta C, Cerretani D, et al. Corneal crosslinking: riboflavin concentration in corneal stroma exposed with and without epithelium. J Cataract Refract Surg, 2009, 35(5): 893- 899. DOI: 10.1016/j.jcrs.2009.01.009.
[26]
Cassagne M, Laurent C, Rodrigues M, et al. Iontophoresis transcorneal delivery technique for transepithelial corneal collagen crosslinking with riboflavin in a rabbit model. Invest Ophthalmol Vis Sci, 2016, 57(2): 594-603. DOI: 10.1167/ iovs.13-12595.
[27]
Mastropasqua L, Nubile M, Calienno R, et al. Corneal crosslinking: intrastromal riboflavin concentration in iontophoresisassisted imbibition versus traditional and transepithelial techniques. Am J Ophthalmol, 2014, 157(3): 623-630.e1. DOI: 10.1016/j.ajo.2013.11.018.
[28]
Ostacolo C, Caruso C, Tronino D, et al. Enhancement of corneal permeation of riboflavin-5'-phosphate through vitamin E TPGS: a promising approach in corneal trans-epithelial cross linking treatment. Int J Pharm, 2013, 440(2): 148-153. DOI: 10.1016/ j.ijpharm.2012.09.051.
[29]
Iselin KC, Thiel MA, Bachmann LM, et al. Fluorophotometric determination of riboflavin concentrations in a human artificial anterior chamber model. Transl Vis Sci Technol, 2019, 8(6): 7. DOI: 10.1167/tvst.8.6.7.
[30]
Lombardo G, Micali NL, Villari V, et al. All-optical method to assess stromal concentration of riboflavin in conventional and accelerated UV-A irradiation of the human cornea. Invest Ophthalmol Vis Sci, 2016, 57(2): 476-483. DOI: 10.1167/ iovs.15-18651.
[31]
Lombardo G, Micali NL, Villari V, et al. Assessment of stromal riboflavin concentration-depth profile in nanotechnology-based transepithelial corneal crosslinking. J Cataract Refract Surg, 2017, 43(5): 680-686. DOI: 10.1016/j.jcrs.2017.03.026.
[32]
Lombardo M, Micali N, Villari V, et al. Ultraviolet A: Visible spectral absorbance of the human cornea after transepithelial soaking with dextran-enriched and dextran-free riboflavin 0.1% ophthalmic solutions. J Cataract Refract Surg, 2015, 41(10): 2283-2290. DOI: 10.1016/j.jcrs.2015.11.007.
[33]
Lombardo M, Lombardo G. Noninvasive real-time assessment of riboflavin consumption in standard and accelerated corneal crosslinking. J Cataract Refract Surg, 2019, 45(1): 80-86. DOI: 10.1016/j.jcrs.2018.07.062.
[34]
Rubinfeld RS, Stulting RD, Gum GG, et al. Quantitative analysis of corneal stromal riboflavin concentration without epithelial removal. J Cataract Refract Surg, 2018, 44(2): 237- 242. DOI: 10.1016/j.jcrs.2018.01.010.
[35]
Vinciguerra P, Mencucci R, Romano V, et al. Imaging mass spectrometry by matrix-assisted laser desorption/ionization and stress-strain measurements in iontophoresistransepithelial corneal collagen cross-linking. Biomed Res Int, 2014, 2014: 404587. DOI: 10.1155/2014/404587.
浙公网安备 33030202001892号