ANetwork Pharmacological Study on the Targets ofAction and Related Mechanisms of Lycium Barbarum in the Treatment of Dry Eye
Qing Gao1, Qingbao Pang1, He Sun2
1Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
2The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
Abstract：Objective: Anetwork pharmacology-based approach to investigate the targets and mechanisms of action of Lycium barbarum in the treatment of the dry eye. Methods: In July 2022, the chemical composition of Lycium barbarum was collected through the TCMSP database, and the active ingredients were screened. The OMIM and GeneCards databases were used to obtain the target genes related to dry eye, and the common target of "Lycium barbarum-dry eye" was obtained as the key gene through Venny diagram. The STRING database was used to construct the "disease-traditional Chinese drug-component-target" network and the protein-protein interaction (PPI) network, and the core genes were obtained based on the Degree algorithm by using Cytoscape 3.9.0 software. Gene ontology (GO) and the Kyoto encyclopedia of genes and genomes (KEGG) were conducted into analysis. Results: Lycium barbarum screened 31 active compounds with 45 targets; dry eye screened 875 target genes and obtained 403 intersecting genes after taking the intersection. The top 12 core proteins were HIF1A, EGFR, IL-6, MYC, VEGFA, CASP3, CCND1, ERBB2, PPARG, FOS, NFKBIA, CASP8, etc. Seventy-one GO function enrichment results were obtained, mainly related to the regulation of cellular infammatory factors, apoptosis, immune response, etc. KEGG pathway enrichment results totaled 97, mainly related to lipid and atherosclerosis, cancer pathways, chemo-oncogenic-receptor activation, Kaposi's sarcoma-associated herpesvirus infection, PI3K-Akt, NF-kappa-B, VEGF, AGE-RAGE, and other signaling pathways. Conclusions: Lycium Barbarum may be used to reduce the ocular surface infammatory response and improve symptoms and clinical indices through the effects on genes such as EGFR, IL-6, VEGFA, CASP3 and the modulation of signaling pathways such as cancer pathway, PI3K-Akt, NF-kappa-B, VEGF,AGE-RAGE.
高青, 庞庆宝, 孙河. 枸杞子治疗干眼症的作用靶点及相关机制的网络药理学研究[J]. 中华眼视光学与视觉科学杂志, 2023, 25(5): 340-346.
Qing Gao1, Qingbao Pang1, He Sun2. ANetwork Pharmacological Study on the Targets ofAction and Related Mechanisms of Lycium Barbarum in the Treatment of Dry Eye. Chinese Journal of Optometry Ophthalmology and Visual science, 2023, 25(5): 340-346. DOI: 10.3760/cma.j.cn115909-20220813-00322
浙公网安备 33030202001892号