Objective To study the expression of inflammatory factors IL-17A， IL-1β， IL-6 and TNF-α in the lachrymal glands of ovariectomized rats when the level of sex hormones changes. Methods This was an experimental study. Twenty healthy female SD rats were divided randomly into a control group （n=10） and an experimental group （n=10）. Ovariectomy was performed on the experimenal group， while a sham operation was performed on the control group. A Schirmer Ι test （SIT） and corneal fluorescence staining were performed on both groups before surgery and at 1， 2， and 3 months after surgery. The results were analyzed with the repeated measurement ANOVA. Acinar cells from the lacrimal glands were examined by HE staining. At the 3rd month， the concentrations of estrogen and androgen were measured by radiation immunoassay detection and were analyzed with independent sample t tests. The expressions of IL-17A， IL-1β， IL-6 and TNF-α were analyzed by immunohistochemical and Western blot methods with independent sample t tests. Results The concentration of estrogen and androgen in the experimental group decreased compared with the control group （t=-35.37， -12.13， P<0.01）. There were no differences between the control group and the experimental group for the SIT and corneal fluorescence staining at each time point （P>0.05）. HE staining showed that the enzymes of the original particles in the lachrymal cells were significantly reduced while acinar atrophy and cell arrangement disorder were observed in the experimental group. Immunohistochemical analysis showed that the expression of inflammatory factors in the lachrymal glands was significantly higher in the experimental group compared with those in the control group （A value： IL-17A： t=7.56， P<0.01， IL-1β： t=13.71， P<0.01， IL-6： t=13.92， P<0.01， TNF-α： t=6.11， P<0.01）. Western blot analysis showed that the expression levels of inflammatory factors in the lachrymal glands were significantly higher in the experimental group compared with those in the control group （A value： IL-17A： t=20.90， P<0.01， IL-1β： t=16.93， P<0.01， IL-6： t=12.46， P<0.01， TNF-α： t=14.47， P<0.01）. Conclusion The levels of estrogen and androgen in the experimental group dropped at the 3rd month postoperatively but there were no obvious clinical signs found on the ocular surface. The expression of inflammatory factors， such as IL-17A， IL-1β， IL-6 and TNF-α， increased in the lachrymal glands of ovariectomized rats.
钟蕾,张晓峰,夏静,夏蔚,孙正太,王英明. 去卵巢对大鼠泪腺中IL-17A等炎症因子表达的影响[J]. 中华眼视光学与视觉科学杂志, 2014, 16(9): 522-526.
Zhong Lei,Zhang Xiaofeng,Xia Jing,Xia Wei,Sun Zhengtai,Wang Yingming. Expression of IL-17A in the lachrymal glands of rats after ovariectomy. Chinese Journal of Optometry Ophthalmology and Visual science, 2014, 16(9): 522-526. DOI: 10.3760/cma.j.issn.1674-845X.2014.09.003
Guillon M， Malssa C. Tear film evaporation—effect of age and gender [J]. Cont Lens Anterior Eye， 2010， 33：171-175.
[2]
Scuderi G， Contestabile MT， Gagliano C， et al. Effects of phytoestrogen supplementation in postmenopausal women with dry eye syndrome： a randomized clinical trial[J]. Can J Ophthalmol， 2012， 47：489-492.
[3]
Jonsson R， Vogelsang P， Volchenkov R， et al. The complexity of Sjögren′s syndrome： novel aspects on pathogenesis[J]. Immunol Lett， 2011， 141：1-9.
[4]
Sullivan DA. Tearful relationships? Sex， hormones， the lacrimal gland， and aqueous-deficient dry eye[J]. Ocul Surf，2004，2：92-123.
Mostafa S， Seamon V， Azzarolo AM. Influence of sex hormones and genetic predisposition in Sjögren′s syndrome： a new clue to the immunopathogenesis of dry eye disease[J]. Exp Eye Res，2012，96：88-97.
[7]
Fujihara T， Murakami T， Fujita H， et al. Improvement of corneal barrier function by the P2Y（2） agonist INS365 in a rat dry eye model[J]. Invest Ophthalmol Vis Sci，2001，42：96-100.
[8]
Park CY， Zhuang W， Lekhanont K， et al. Lacrimal gland inflammatory cytokine gene expression in the botulinum toxin B-induced murine dry eye model[J]. Mol Vis，2007，29：2222-2232.
[9]
Corcoran MP， Meydani M， Lichtenstein AH， et al. Sex hormone modulation of proinflammatory cytokine and creactive protein expression in macrophages from older men and postmenopausal women[J]. J Endocrinol，2010，206：217-224.
[10]
Barabino S， ChenY， Chauhan S， et al. Ocular surface immunity： homeostatic mechanisms and their disruption in dry eye disease[J]. Prog Retin Eye Research，2012，31：271-285.
Miossec P， Kolls JK. Targeting IL-17 and TH17 cells in chronic inflammation[J]. Nat Rev Drug Discov，2012，11：763-776.
[13]
Humphreys-Beher MG， Hu Y， Nakagawa Y， et al. Utilization of the non-obese diabetic （NOD） mouse as an animal model for the study of secondary Sjögren′s syndrome[J]. Adv Exp Med Biol，1994，350：631-636.
[14]
Haneji N， Hamano H， Yanagi K， et al. A new animal model for primary Sjögren′s syndrome in NFS/sld mutant mice[J]. J Immunol，1994，153：2769-2777.
浙公网安备 33030202001892号