Objective To explore the influence of head tilt on fovea-disc angle （FDA）. Methods This was a self-controlled study. 3D OCT-1000 was used to take fundus photos for 26 non-strabismus subjects （52 eyes） at entopic position， head to the right at 30° and head to the left at 30° respectively. CorelDRAW X4 drawing software was used to measure the FDA values in these photos. The data were analyzed using t test， analysis of variance and linear regression. Results FDA values： the differences in right eye among head to the right （13.53°±3.36°）， entopic position （6.56°±2.28°） and head to the left （-0.53°±3.88°） had statistical significance （F=115.866， P<0.05）. The FDA value of head to the right was significantly higher than that of the entopic position （P<0.05）， while the FDA value of the entopic position was significantly higher than head to the left （P<0.05）. The differences in left eye between head to the right （1.08°±3.31°）， entopic position （7.31°±2.80°） and head to the left （13.45°±3.44°） had statistical significance （F=98.437， P<0.05）. The FDA value of head to the right was significantly lower than that of the entopic position （P<0.05）， while the FDA value of the entopic position was significantly lower than head to the left （P<0.05）. There were no significant differences between the FDA values of different genders （t=0.906， P>0.05）. The FDA values were not correlated with age （r=0.001， P>0.05）. Conclusion Head tilt has a significant influence on fovea-disc angle. In clinical practice， when fundus photography is used to evaluate the fundus rotation of patients， attention should be paid to the placement of head.