Objective To investigate the effect of peroxisome-proliferator-activated receptor-γ coactivator-1α （PGC-1α） on vascular endothelial growth factor （VEGF） expression and tube formation in human vascular endothelial cells. Methods This was an experimental study. Recombinant PGC-1α protein was added to human vascular endothelial cells （HUVEC）， and no recombinant PGC-1α protein was added to HUVEC for the control group. After 24 hours of incubation， the two groups of cells were placed into a normoxic （20% O2） or hypoxic （1% O2） environment for another 16 hours. The expression of VEGF mRNA and protein were detected by real-time PCR and ELISA. After 48 hours of incubation， the two groups of cells were plated onto Matrigel-coated plates and cultured under normoxic or hypoxic conditions for another 48 hours. The tube formation of cells was observed by optical microscopy. Experimental data were analyzed by one-way ANOVA. Results VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC-1α protein both under normoxia and hypoxia conditions compared to the control groups （F=131.67， 65.96， P<0.01）. The tube formation ability of cells was enhanced significantly after treatment with recombinant PGC-1α protein compared to the control groups under both normoxia and hypoxia conditions. Conclusion PGC-1α can upregulate the expression of VEGF and promote tube formation in HUVEC.
蒋剑,夏晓波,张露. PGC-1α对人血管内皮细胞VEGF表达及管腔形成的调控[J]. 中华眼视光学与视觉科学杂志, 2015, 17(5): 280-283.
Jiang Jian,Xia Xiaobo,Zhang Lu. Regulation of VEGF expression and tube formation in human vascular endothelial cells by PGC-1α. Chinese Journal of Optometry Ophthalmology and Visual science, 2015, 17(5): 280-283. DOI: 10.3760/cma.j.issn.1674-845X.2015.05.006
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