Objective To observe the matrix metalloproteinase 2 (MMP-2) activity and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to an extremely low frequency electromagnetic field (ELF-EMF) and its impact on collagen I synthesis. Methods This was an experimental study. HFSFs were cultured and divided into a control group, radiation group and inhibitor group according to their exposure to 50 Hz, 0.2 mT electromagnetic field or joint MMP-2 specific inhibitors (sc-204092). MMP-2 activity was measured by gelatin enzymography. MMP-2 and collagen I protein expression were detected by Western Blot. A single factor analysis of variance was used to determine whether there was a difference among these groups. Results There were significant differences between the three groups (F=14.96, 139.88, 63.46, P<0.01). Compared to the control group, MMP-2 activity and protein expression increased in the radiation group (P<0.05) and collagen I protein expression decreased (P<0.01). Compared to the radiation group, MMP-2 activity and protein expression decreased in the inhibitor group (P<0.01) and collagen I protein expression increased (P<0.05). Conclusion ELF-EMF may regulate the synthesis of collagen I by affecting the activity and protein expression of MMP-2.
田甜,朱煌. 极低频电磁辐射下HFSF中MMP-2的表达及作用[J]. 中华眼视光学与视觉科学杂志, 2015, 17(6): 331-334.
Tian Tian,Zhu Huang. Expression and effect of matrix metalloproteinase 2 in human fetal scleral fibroblasts treated with an extremely low frequency electromagnetic field. Chinese Journal of Optometry Ophthalmology and Visual science, 2015, 17(6): 331-334. DOI: 10.3760/cma.j.issn.1674-845X.2015.06.004
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