Objective To investigate the status of bacterial flora in the conjunctival sac of adolescents wearing orthokeratology lenses and in their storage cases. Methods One hundred and one adolescents wearing orthokeratology lenses （wearing group） and 110 adolescents who do not wear the lenses （control group） were included in the survey. There were 158 storage cases joined in the test. Samples collected from the conjunctival sacs and storage cases were cultured at 37 ℃ for 48 h. Results Fifty-nine strains of bacteria were cultured in the wearing group， with a positive rate of 29.4%. In the control group， 68 strains were found， and the positive rate was 30.9%. There was no significant difference between the two groups （χ²=0.12， P>0.05）. Seventy-two strains of bacteria were cultured in the orthokeratology lens storage cases， with a positive rate of 45.6%. Staphylococcus epidermidis was the most prevalent bacteria in the bacterial flora and storage cases of the two groups. Conclusion Wearing orthokeratology lenses does not significantly increase the positive rate of bacterial flora. At the same time， cleaning lenses and cases is effective in reducing the rate of lens pollution.
王英明,张晓峰,钱雪峰,夏蔚,钟蕾,孙正太,付心怡. 青少年角膜塑形镜配戴者结膜囊及镜盒菌群研究[J]. 中华眼视光学与视觉科学杂志, 2015, 17(10): 614-617.
Wang Yingming,Zhang Xiaofeng,Qian Xuefeng,Xia Wei,Zhong Lei,Sun Zhengtai,Fu Xinyi. Research on bacterial flora in the conjunctival sacs and storage cases of adolescent wearing orthokeratology lenses. Chinese Journal of Optometry Ophthalmology and Visual science, 2015, 17(10): 614-617. DOI: 10.3760/cma.j.issn.1674-845X.2015.10.009
Szczotka-Flynn LB， Pearlman E， Ghannoum M. Microbial contamination of contact lenses， lens care solutions， and their accessories： a literature review[J]. Eye Contact Lens，2010，36（2）：116-129.
[2]
Goldstein MH， Kowalski RP， Gordon YJ. Emerging fluoroquinolone resistance in bacterial keratitis： a 5-year review[J]. Ophthalmology，1999，106（7）：1313-1318.
[3]
Speaker MG， Milch FA， Shah MK， et al. Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis[J]. Ophthalmology，1991，98（5）：639-650.
[4]
Erdo■an H， Kemal M， Toker MI， et al. Effect of frequent-replacement contact lenses on normal conjunctival flora[J]. CLAO J，2002，28（2）：94-95.
[5]
Rubio EF. Influence of age on conjunctival bacteria of patients undergoing cataract surgery[J]. Eye，2006，20（4）：447-454.
[6]
Iskeleli G， Bahar H， Eroglu E， et al. Microbial changes in conjunctival flora with 30-day continuous-wear silicone hydrogel contact lenses[J]. Eye Contact Lens，2005，31（3）：124-126.
[7]
Lam PT， Hui M， Young AL， et al. Preoperative antisepsis with povidone-lodine 5% in cataract surgery[J]. Asia Pac J Ophthalmol，2012，1（2）：77-83.
[8]
Ueta M， Iida T， Sakamoto M， et al. Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface[J]. J Med Microbiol，2007，56（Pt 1）：77-82.
[9]
Larkin DF， Leeming JP. Quantitative alterations of the commensal eye bacteria in contact lens wear[J]. Eye，1991，5（Pt 1）：70-74.
[10]
Donzis PB， Mondino BJ， Weissman BA， et al. Microbial contamination of contact lens care systems[J]. Am J Ophthalmol，1987，104（4）：325-333.
Hayashi S， Suzuki T， Yamaguchi S， et al. Genotypic characterization of staphylococcus aureus isolates from cases of keratitis and healthy conjunctival sacs[J]. Cornea，2014，33（1）：72-76.
[14]
Inagaki K， Yamaguchi T， Ohde S， et al. Bacterial culture after three sterilization methods for cataract surgery[J]. Jpn J Ophthalmol，2013，57（1）：74-79.
[15]
Dutta D， Cole N， Willcox M. Factors influencing bacterial adhesion to contact lenses[J]. Mol Vis，2012，18（3）：14-21.
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