Objective To develop and test a Chinese version vision-relatde quality of life questionnaire-25. Methods In this investigtion study, the National Eye Institute Visual Function Questionnaire (NEI VFQ-25) was translated and adapted into the Chinese version vision-related quality of life questionnaire (CVRQoL-25). The questionnaire items were completed by 113 patients with age-related cataract, hypermyopia, glaucoma, diabetic retinopathy and by a reference group (n=38). The rates of missing data, Cronbach′ alpha coefficient, content validity, construct validity and discriminant validity of CVRQoL-25 were computed. The results were compared with patient demographics multivariate analysis of variance, factor analysis and Pearson correlation were used for statistical analysis. Results Based on the information from the participants, one item-“Driving” was changed to conclude driving cars and riding bicycles. At scale “Driving”, the rate of missing data was very high both in patient and control groups, while all the other scales were responded to each member in the research. Cronbach′ alpha coefficient for the ten scales and the total CVRQoL-25 scale were above 0.6. There was significant difference between the two groups. The content validity of all scales were above 0.4 and each scale was included in one of the major factors. The scores of CVRQoL-25 were highly correlated with visual acuity of the better eye, both eye, and with education. Conclusion With minor modifications from the English version, CVRQoL-25 can be used as one measure instrument to evaluate the vision-related quality of life of patients with eye disease. The scores of CVRQoL-25 had no significant relations with sex, and had negtive relations with age. Subjects who with higher education degree had higher scores of CVRQoL-25.