Application of the Bicinchoninic Acid Method to Quantitatively Detect Residual Medical Multi-Enzyme Detergent
Yuan Tang1 , Dan Li2 , Hongmei Qian1 , Yanyan Chen1, 3, Lei Qin4 , Zailong Chi1, 3
1 School of Ophthalmology and Optometry, Biomedical Engineering, Wenzhou Medical University, Wenzhou 325027, China 2 School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325027, China 3 State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China 4 Ningbo College of Health Sciences, Ningbo 315000, China
Abstract： Objective: To establish a quantitative determination method for medical multi-enzyme detergent. Methods: This was an experimental study. A quantitative method for the determination of medical multienzyme detergent was established by using the bicinchoninic acid (BCA) method, and its reproducibility, accuracy and recovery were determined. Results: The standard curve, equation and the data analysis method of the samples were established. The BCA standard curve was very good and has been repeated in experiments, R2 >0.95. The standard protein recovery test showed that the recovery rate was <100±15% and the relative standard deviation (RSD) was <3%. BCA method was used to determine the multi-enzyme detergent and multi-enzyme detergent of the 1∶50 and 1∶100 dilutions, RSD<5%. The multi-enzyme detergent stock solution was diluted into six concentration gradients. The results of BCA showed that the concentration of enzyme protein decreased with an increase in the dilution ratio in the range of 1∶50-1∶200. Conclusions: The BCA method can be used for the quantitative determination of multi-enzyme detergents for medical devices with good sensitivity, recovery, stability and accuracy.
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