Objective Investigate the influence for accommodation status and discuss the relationship between the accommodation status and asthenopia after laser in situ keratomileusis (LASIK).Methods In this retrospective study,20 emmetropes and 60 myopes (SE=-5.52±1.60 D,astigmatism ＜2.00 D,BCVA≥1.0) experienced LASIK from March 2009 to August 2009 aged 18～35 years old were selected form the Department of Ophthalmology in DongFeng General Hospital.Twenty emmetropes were selected as control.Monocular and binocular accommodative amplitudes for all people before surgery, 1 week,2 week, 1 month after surgery were surveyed,while symptoms of asthenopia were evaluated by questionnaire.Independent samples t test,ANOVA and Pearson correlation were used to analyze the data.Results ①Accommodation status:monocular and binocular accommodative amplitudes were (7.82±0.58)D and (8.10-±O.54)D 1 week after LAS1K,descended compared to preoperation (P＜O.05),while recovered and surpassed the preoperative level at 1 month after LASIK, and there was no significant difference between the myopes and emmetropes after 1 month.②Symptoms of asthenopia:the asthenopia symptoms of myopes aggravated significantly during the first week after LASIK, and recoverd at 1 month after LASIK.③Correlation analysis showed that the symptoms of asthenopia has a negative correlation with binocular accommodative amplitude in the first week after surgery (r=-0.79,P＜0.01).The descending extent of the accommodative amplitude had positive correlation with the refractive power before surgery (r=0.83,P＜0.01).Conclusion After LASIK, the accommodative amplitude appears to decrease temporarily,especially in the first week after LASIK,and then recovered progressively; the demand of accommodation increases after surgery.The symptoms of asthenopia after LASIK are associated with the change of accommodative amplitude.