Objective To investigate the effect of basic fibroblast growth factor (bFGF) on proliferation and migration in cultured human lens epithelial cell (HLEC). Methods HLEC cultured in a serum-free medium were treated with bFGF (0.01, 0.1, 1, 10 and 100 μg/L). MTT assay was used to detect the proliferation effect and a FACS machine was used to detect the cell cycle. An in vitro wound healing model was used to detect the migration of HLEC treated with bFGF (0.01, 0.1, 1, 10 and 100 μg/L) after 24 hours. Results bFGF (0.1, 1, 10, 100 μg/L) increased proliferation rates of cultured HLEC with density/time dependence. Compared to the control group, there was a significant difference (P<0.01). The addition of bFGF at a concentration of 100 μg/L for 24 hours was the maximal increase in the proliferation rate (112.78%). bFGF also induced proliferation by stimulating the G0/G1 phase cell decrease and increasing the S phase and G2/M phase. bFGF showed effects on migration of 27.21%, 154.42%, and 238.77% at concentrations of 1 μg/L, 10 μg/L. 100 μg/L, respectively. Compared to the control group, there was a significant difference (P <0.01). Conclusion bFGF can induce the proliferation and obvious migration of HLEC; consequently, bFGF is a mitogen and potent migratory factor for HLEC.
哈玲芳,盛迅伦,庄文娟,武淑玲. 碱性成纤维细胞生长因子对人晶状体上皮细胞增殖及移行作用的影响[J]. 中华眼视光学与视觉科学杂志, 2010, 12(2): 134-137.
HA Ling-fang,SHENG Xun-lun,ZHUANG Wen-juan,WU Shu-ling. Effect of basic fibroblast growth factor on proliferation and migration in cultured human lens epithelial cells. Chinese Journal of Optometry Ophthalmology and Visual science, 2010, 12(2): 134-137. DOI: 10.3760/cma.j.issn.1674-845X.2010.02.014