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| The regulation of human fetal scleral fibroblasts using soluble guanylyl cyclase antagonist in vitro |
| Li Chuang*, Lyu Yong, Zhao Hong |
| * Department of Ophthalmology, Zhengzhou Second Hospital, Zhengzhou 450006, China |
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Abstract Objective To study scleral fibroblast proliferation and type I collagen compound in human fetal scleral fibroblasts (HFSFs) by applying the soluble guanylyl cyclase (sGC) antagonist (NS-2028). Methods Experimental study. HFSFs were divided into two groups. The experimental group was treated with NS-2028; the control group was not treated. The experimental concentrations were 0.1, 1.0, 10.0, 100.0 μmol/L. After NS-2028 stimulation, the changes in the HFSFs of the control group and experimental groups were analyzed with CCK8, realtime fluorescence quantitative PCR (RTFQ PCR) and immunofluorescence (IF). Results ①The OD value of HFSFs in the control group was 0.82±0.04. The OD values of the four NS-2028 treated groups were 0.81±0.05, 0.83±0.05, 0.85±0.05 and 0.81±0.05, there were no significant differences from the control group (F=0.620, P>0.05). ②The relative transcript levels of COL1A1 mRNA in the the four NS-2028 treated groups were 1.06±0.75, 1.19±0.68, 1.40±0.18, 1.88±0.24, there were significant differences compared to the control group(F=32.138, P＜0.05). ③The relative expression level of COL1A1 in HFSFs increased in the four NS-2028 treated groups compared to the control group. Conclusion NS-2028 plays a significant role in promoting the composition of COL1A1. There may be no significant difference in the proliferation of HFSFs. However, COL1A1 may directly affect HFSFs, delaying the development of myopia.
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Corresponding Authors:
Lyu Yong, Email: lyong@zzu.edu.cn
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