Objective To study the correlation between the stimulus and response AC/A ratios by measuring a patient′s accommodative lag and eye position using different stimuli to induce accommodation. Methods A cross-sectional study was used. Twenty-nine myopic college students（average age 23.3±3.8） were randomly selected for the study. Phoria was measured at distances of 40 cm and 5 m by prism separation when wearing full correction. Phoria was measured again at a distance of 40 cm after adding spherical lenses of +1.00 D， +1.50 D， -1.00 D and -2.00 D. Accommodative lag was then measured with each lens power by the monocular estimation method using dynamic retionscopy. Finally， the data were analyzed by a rank-sum test. Results There were statistically significant differences between accommodative lag and gradient response and stimulus AC/A ratios when different accommodative stimuli were used （x²=116.85， 8.992， 17.319， P<0.05）. The correlation coefficients between response and stimulus AC/A ratios based on the gradient method were 0.542（P<0.01）， 0.789（P<0.01），and 0.812（P<0.01） with added lenses of +1.00 D， -1.00 D， -2.00 D. And the correlation coefficient between response and stimulus AC/A ratios based on the calculation method was 0.859（P<0.01）. Conclusion A subject has less accommodative response and more accommodative lag with minus spherical addition than with a plus spherical addition lens. The correlation between stimulus and response AC/A ratios is highest when using a calculation method.