基于网络药理学和生物信息学探讨龙胆泻肝汤治疗葡萄膜炎的分子机制

doi:10.3760/cma.j.issn.1674-845X.2018.08.006.

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Abstract

Objective: To study the molecular mechanism of LongdanXiegan Tang for treating uveitis. Methods:This was a experimntal study. The Chinese herbal medicine pharmacology data and analysis platform were used to search and to screen the effective components of a compound injection of LongdanXiegan Tang, and to analyze its possible therapeutic targets based on the network topology. By searching a variety of known disease target databases, we can screen the therapeutic target of uveitis disease and construct the active ingredient-disease treatment target network with the target of effective component treatment and to screen out the key nodes. Using topological analysis of the key nodes involved in the signalpathways and enrichment analysis, and screening out the possible Chinese medicine compound Radix LongdanXiegan Tang for the treatment of uveitis, may help to identify the signal pathways. Results: After the main active ingredient for screening and target prediction of LongdanXiegan Tang were completed, the therapeutic target of uveitis was screened, and an enrichment analysis of the signal pathways was completed. LongdanXiegan Tang can reduce the production of inflammatory cytokines and infiltration and affect the immune status, regulating the occurrence and development of uveitis by mainly regulating the MAPK signaling pathway and promoting both the GnRH and estrogen signaling pathways. Conclusions: LongdanXiegan Tang can treat uveitis primarily through the MAPK, GnRH and estrogen signaling pathways, regulating the inflammatory cells and inflammatory cytokines and autoimmunity.

Key words： LongdanXiegan Tang uveitis traditional Chinese medicine compound network pharmacology

Received: 19 April 2018

Fund:

National Natural Science Foundation (81674032, 81373826); Projects of Medical and Health Technology Development Program in Shandong Province (2015-147)

Corresponding Authors: Hongsheng Bi, Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Shandong Provincial Universities' Key Laboratory of Prevention and Therapy of Ocular Diseases, Jinan 250002, China (Email: hongshengbi1@126.com);Lijie Li, Institute of Endocrinology and Metabolism in Shandong, Jinan 250062, China (Email: yankeboshi@163.com)

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https://www.cjoovs.com/EN/10.3760/cma.j.issn.1674-845X.2018.08.006. OR https://www.cjoovs.com/EN/Y2018/V20/I8/484

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