蛋白酶体β5过表达对人晶状体上皮细胞抗氧化能力的影响

doi:10.3760/cma.j.cn115909-20190829-00236

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Abstract Objective: To discuss the antioxidative ability of proteasome subunit β5 (PSMB5) over expression in human lens epithelial cells. Methods: This is an experimental study. Recombinant plasmid pcDNA3.1- PSMB5 was constructed and transfected into a human lens epithelium strain SRA01/04 to form a stable transfection (experimental group), and empty pcDNA3.1 was also transfected into lens epithelial cells at the same time as a control. Two groups of cells were treated with 40 μmol/L H2O2. The nuclear morphology of the two group cells was observed by Hoechst 33342 stain, and cell apoptosis of the two group cells was dectected by flow cytometry. Data was analyzed by a paired t test. Results: Under a 40 μmol/L H2O2 treated environment, Staining by Hoechst 33342 indicated that the nuclei in the control group were significantly shrunken and condensed compared with those of the experimental group. The apoptotic percentages detected by flow cytometry in the experimental group and control group were (9.3±0.9)% and (15.7±1.9)%,and both had increased compared to pre-treatment with H2O2, which were (5.9±0.8)% and (7.1±1.1)%, respectively. The apoptotic percentage in the control group increased to a higher level than in the experimental group (t=3.742, P=0.008). Conclusions: Overexpression of proteasome PSMB5 can effectively improve the antioxidative ability of human lens epithelial cells.

Key words： overexpression proteasome subunit β5 proteasome lens antioxidative

Received: 29 August 2019

Fund:Guangzhou Medical and Health Science Reseach Foundation (20171A010336)

Corresponding Authors: Tieying Zhang, Department of Ophthalmology, Affiliated Hexian Memorial Hospital, Southern Medical University, Hexian Memorial Medical Hospital of Panyu District, Guangzhou 511400, China (Email: rosezhang1976@163.com)

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