不同浓度鼠神经生长因子对兔眼LASIK术后角膜神经修复的影响

doi:10.3760/cma.j.cn115909-20191018-00282

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Abstract Objective: To investigate the effect of different concentrations of mouse nerve growth factor (mNGF) in eye drops on rabbits' central corneal nerve regeneration after laser in situ keratomileusis. Methods: In this experimental study, 39 New Zealand white rabbits were included and randomly assigned to 5 groups: normal saline group (normal saline, 7 rabbits, 14 eyes), hycosan group (0.1% hycosan, 8 rabbits, 16 eyes), NGF50 group (50 µg/mL, 8 rabbits, 16 eyes), NGF100 group (100 µg/mL, 8 rabbits, 16 eyes) and NGF200 group (200 µg/mL, 8 rabbits, 16 eyes). Central corneal subbasal nerve densities (SNDs) and superficial stroma nerve densities (SSNDs) were measured by confocal microscopy Ⅲ and were compared before surgery, 1 week, 1 month, 2 months and 3 months after surgery. Repeated measures and one-way analysis of variance were used for comparing the differences between different time points for these groups. Results: The differences in preoperative SNDs and SSNDs in the normal saline group, hycosan group, NGF50 group, NGF100 group and NGF200 group were not statistically significant (F=1.371, P=0.252; F=0.372, P=0.828). Compared with preoperative values, all nerve parameters of the 5 groups decreased at 1 week (all P<0.05) postoperatively, but the differences among the 5 groups were not statistically significant (F=1.905, P=0.119; F=0.923, P=0.455). SNDs in the NGF100 group and NGF200 group were significantly higher than in the other 3 groups at 1 month after surgery (all P<0.05); SNDs in the NGF200 group were significantly higher than in the other groups at 2 months and 3 months postoperatively (all P<0.05). SSNDs in the NGF200 group were significantly higher than in the other groups at 1 and 2 months after surgery (all P<0.05). At 3 months after surgery, SSNDs in the NGF200 group were significantly higher than those in the NGF50 group and the 2 nonNGF groups (all P<0.05). The NGF50 group and NGF100 group had higher densities than the 2 non-NGF groups (all P<0.05). Conclusions: mNGF eye drops have a significant effect on promoting corneal nerve repair in different periods following LASIK, and the effect is highest in the NGF200 group.

Key words： laser in situ keratomileusis nerve growth factor corneal nerve regeneration dose dependent effect

Received: 18 October 2019

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Fund: Wenzhou Science and Technology Plan Project (Y20160442); Natural Science Foundation of Zhejiang Province (LY17H120005)

Corresponding Authors: Liang Hu, Eye Hospital of Wenzhou Medical University, Wenzhou 325027, China (Email: liang_hu@live.cn)

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https://www.cjoovs.com/EN/10.3760/cma.j.cn115909-20191018-00282 OR https://www.cjoovs.com/EN/Y2020/V22/I9/683

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