离体及活体念珠菌在共聚焦显微镜下的特征

doi:10.3760/cma.j.cn115909-20200420-00160

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Abstract Objective: To use in vivo laser confocal microscopy (IVCM) to observe the in vitro culture and living characteristics of candida spores and pseudohyphaes, and to provide abasis for the early diagnosis of candida keratitis. Methods: The data of 26 patients (26 eyes) who were diagnosed with candida keratitis in the Eye Hospital of Shandong First Medical University from January 2016 to December 2019 was collected. At the same time, candida albicans and candida parapsilosis were taken for laboratory fungal culture, and the typical morphology of candida spores and pseudohyphae in vitro and in vivo were observed by IVCM. Results: Candidaspores in vitro and in vivo presented uniformly in the shape of round spots with a diameter of 2-5 μm and a clear boundary. Pseudohyphae have a highly reflective linear structure. The reflection of spores was significantly enhanced in vivo. Eighteen eyes showed a scattered arrangement of spot-like, strongly reflective structure, and 11 eyes showed a dense fog structure. The morphology of pseudomycelia varied greatly. The mycelia in 2 eyes were slender, the spore structure was present in 5 eyes, and branches were visible. In 1 eye, an irregular thin and long rod shape with high contrast was observed, and in another eye, an irregular thin and long rod shape and spore structure could be observed simultaneously. Candida albicans pseudohyphae were relatively slender, with a length of 20-150 μm and a diameter of 2-10 μm. Pseudohyphae of candida parapsilosis were short and thick, with a length of about 30-100 μm and a diameter of 2-5 μm. Part of the branch spore structure was highlighted. Conclusions: Candida keratitis showstypical spore and pseudohyphae structures with IVCM. The morphology of candida keratitis spores in vitro and in vivo are consistent with observations under IVCM, but the morphology of pseudohyphaes are relatively different. IVCM can be used for the early diagnosis of candida keratitis.

Key words： candidas spore pseudomycelial keratitis confocal microscopy

Received: 20 April 2020

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Fund: National Natural Science Foundation of China (81870639, 81900907); Key Project of National Natural Science Foundation of China (81530027); Taishan Scholar Program (20150215, 201812150)

Corresponding Authors: Xiaolin Qi, Eye Hospital of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250021, China (Email: qinglianqxl@163.com)

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https://www.cjoovs.com/EN/10.3760/cma.j.cn115909-20200420-00160 OR https://www.cjoovs.com/EN/Y2020/V22/I11/809

[1]	Kalkanci A, Ozdek S. Ocular fungal infections. Curr Eye Res, 2011, 36(3): 179-189. DOI: 10.3109/02713683.2010.533810.
[2]	Satpathy G, Ahmed NH, Nayak N, et al. Spectrum of mycotic keratitis in north India: Sixteen years study from a tertiary care ophthalmic centre. J Infect Public Health, 2019, 12(3): 367-371. DOI: 10.1016/j.jiph.2018.12.005.
[3]	McSwiney TJ, Knowles SJ, Murphy CC. Clinical and microbiological characteristics of Moraxella keratitis.?Br J Ophthalmol, 2019, 103(12): 1704-1709. DOI: 10.1136/ bjophthalmol-2018-313557.
[4]	Fontana L, Moramarco A, Mandarà E, et al. Interface infectious keratitis after anterior and posterior lamellar keratoplasty. Clinical features and treatment strategies. A review. Br J Ophthalmol, 2019, 103(3): 307-314. DOI: 10.1136/ bjophthalmol-2018-312938.
[5]	Smith RJ, Maloney RK. Diffuse lamellar keratitis. A new syndrome in lamellar refractive surgery. Ophthalmology, 1998, 105(9): 1721-1726. DOI: 10.1016/S0161-6420(98)99044-3.
[6]	Cruzat A, Qazi Y, Hamrah P. In vivo confocal microscopy of corneal nerves in health and disease. Ocul Surf, 2017, 15(1): 15- 47. DOI: 10.1016/j.jtos.2016.09.004.
[7]	Labbé A, Khammari C, Dupas B, et al. Contribution of in vivo confocal microscopy to the diagnosis and management of infectious keratitis. Ocul Surf, 2009, 7(1): 41-52. DOI: 10.1016/ s1542-0124(12)70291-4.
[8]	Vaddavalli PK, Garg P, Sharma S, et al. Role of confocal microscopy in the diagnosis of fungal and acanthamoeba keratitis. Ophthalmology, 2011, 118(1): 29-35. DOI: 10.1016/ j.ophtha.2010.05.018.
[9]	Chidambaram JD, Prajna NV, Larke NL, et al. Prospective study of the diagnostic accuracy of the in vivo laser scanning confocal microscope for severe microbial keratitis. Ophthalmology, 2016, 123(11): 2285-2293. DOI:10.1016/j.ophtha.2016.07.009.
[10]	Qiu WY, Yao YF. Mycotic keratitis caused by concurrent infections of Exserohilummcginnisii and Candida parapsilosis. BMC Ophthalmol, 2013, 13(1): 37. DOI: 10.1186/1471-2415- 13-37.
[11]	Takezawa Y, Shiraishi A, Noda E, et al. Effectiveness of in vivo confocal microscopy in detecting filamentous fungi during clinical course of fungal keratitis. Cornea, 2010, 29(12): 1346- 1352. DOI: 10.1097/ICO.0b013e3181cd3c84.
[12]	Chidambaram JD, Prajna NV, Palepu S, et al. In vivo confocal microscopy cellular features of host and organism in bacterial, fungal, and acanthamoeba keratitis. Am J Ophthalmol, 2018, 190: 24-33. DOI: 10.1016/j.ajo.2018.03.010.
[13]	Lee JH, Le VH, Lee S, et al. Two-photon microscopy of fungal keratitis-affected rabbit cornea ex vivo using moxifloxacin as a labeling agent. Exp Eye Res, 2018, 174:51-58. DOI: 10.1016/ j.exer.2018.05.018.
[14]	Chidambaram JD, Prajna NV, Larke N, et al. In vivo confocal microscopy appearance of Fusarium and Aspergillus species in fungal keratitis. Br J Ophthalmol, 2017, 101(8): 1119-1123. DOI: 10.1136/bjophthalmol-2016-309656.
[15]	Sedaghat MR, Hosseinpoor SS. Candida albicans interface infection after deep anterior lamellar keratoplasty. Indian J Ophthalmol, 2012, 60(4): 328-330. DOI: 10.4103/0301- 4738.98723.
[16]	Cruzat A, Pavan-Langston D, Hamrah P. In vivo confocal microscopy of corneal nerves: Analysis and clinical correlation. Semin Ophthalmol, 2010, 25(5-6): 171-177. DOI: 10.3109/ 08820538.2010.518133.
[17]	Byrd JA, Caldwell DY, Nisbet DJ. The identification of fungi collected from the ceca of commercial poultry. Poult Sci, 2017, 96(7): 2360-2365. DOI: 10.3382/ps/pew486.
[18]	Le Q, Wu D, Li Y, et al. Early-onset candida glabrata interface keratitis after deep anterior lamellar keratoplasty. Optom Vis Sci, 2015, 92(5): e93-96. DOI: 10.1097/OPX.0000000000000565.
[19]	Lohse MB, Gulati M, Johnson AD, et al. Development and regulation of single- and multi-species Candida albicans biofilms. Nat Rev Microbiol, 2018, 16(1): 19-31. DOI: 10.1038/ nrmicro.2017.107.
[20]	Cruzat A, Pavan-Langston D, Hamrah P. In vivo confocal microscopy of corneal nerves: analysis and clinical correlation. Semin Ophthalmol, 2010, 25(5-6): 171-177. DOI: 10.3109/ 08820538.2010.518133.
[21]	Thomson DD, Wehmeier S, Byfield FJ, et al. Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae. Cell Microbiol, 2015, 17(3): 342-354. DOI: 10.1111/cmi.12369.
[22]	Ledbetter EC, Norman ML, Starr JK. In vivo confocal microscopy for the detection of canine fungal keratitis and monitoring of therapeutic response. Vet Ophthalmol, 2016, 19(3): 220-229. DOI: 10.1111/vop.12287.