角膜胶原交联中核黄素浓度检测方法的研究进展

doi:10.3760/cma.j.cn115909-20200316-00093

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Abstract Corneal collagen cross-linking (CXL) can be used to treat progressive keratoconus, as well as infectious keratitis, keratolysis and corneal stroma edema. At present, riboflavin ultraviolet cross-linking is the commonly used clinical CXL scheme. The concentration and distribution of riboflavin in the cornea is one of the important factors affecting its efficacy and safety. Its main detection methods include fluorescence microscopy imaging, high performance liquid chromatography, spectrophotometry and noninvasive realtime detection of riboflavin concentration, observation of anterior segment slit lamp and imaging mass spectrometry by matrix-assisted laser desorption/ionization, etc. Different detection methods may lead to different results. This paper summarizes riboflavin concentration and its detection methods in current research.

Key words： riboflavin concentration corneal collagen cross-linking confocal microscope high performance liquid chromatography

Received: 16 March 2020

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Fund: Zhejiang Provincial High-Level Talents Program (2017-102); Major Project of the Affiliated Eye Hospital of Wenzhou Medical University (YNZD1201903); Project Launch Fund of Affiliated Eye Hospital of Wenzhou Medical University (KYQD20190701)

Corresponding Authors: Rongrong Gao, Eye Hospital,Wenzhou Medical University, Wenzhou 325027, China (Email: beargrr@163.com)

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https://www.cjoovs.com/EN/10.3760/cma.j.cn115909-20200316-00093 OR https://www.cjoovs.com/EN/Y2021/V23/I8/636

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