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| The Distribution and Expression of 11β-Hydroxysteroid Dehydrogenase
Type 1 in Rat Eyes of Different Ages |
| Xin'an Chen 1, Yong Lin 2, Juxiu Ye |
| 1School of Basic Medical Science, Wenzhou Medical Universtiy, Wenzhou 325035, China
2State Key Laboratory of Ophthalmology and Visual Science, Wenzhou Medical Universtiy, Wenzhou325027, China |
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Abstract Objective: To observe the distribution and expressive changes of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in different age’s rat eyes. Methods: In this experimental research, according to age, SD rats were divided into 6 groups with 6 subjects in each: postnatal days 1-2 (PND1) group, postnatal days 5-6(PND5) group, postnatal days 10-11 (PND10) group, postnatal days 14-15 (PND15) group, postnatal days 20-21 (PND20) group, and 8-9 weeks mature adults rats (M) group. Immunohistochemistry (IHC) assay was used to detect the distribution of 11β-HSD1 protein in the eyes of SD rats at different developmental stages from neonatal, postnatal eyelid opening to mature adult, and the expressions of 11β-HSD1 mRNA and protein levels in the retina or cornea were further verified by real time RT-PCR and Western blot. The data were analyzed by one-way ANOVA or independent sample t-test. Results: 11β-HSD1 protein was widely expressed in such as cornea, iris, retina, choroid, lens, ciliary body of the different age’s rat eyes by IHC staining, and there was more 11β-HSD1 positive cells in neonatal rat eyes. Real time RT-PCR showed that the relative expression of mRNA of 11β-HSD1 peaked at postnatal days 10 in the cornea and decrease gradually after eyelid opening of SD rats (F=8.40, P<0.001); the relative expression of 11β-HSD1 mRNA in the retina of SD rats before eyelid opening was significantly higher than that of eyelid opening rats and adult rats (F=83.50, P<0.001). It was confirmed the relative expression of 11β-HSD1 protein was significantly higher in the retina of neonatal rats than that of eyelid opening rats and mature adult rats by western blot (t=4.76, P=0.009; t=4.24, P=0.013). Conclusion: 11β-HSD1 is widely distributed in SD rat eyes of different ages. The level of 11β-HSD1 expression varies in different age groups. It tends to be higher in eyes of SD rat before eyelid opening. The study suggests that 11β-HSD1 may play an important role in the differentiation, development and maturation of the SD rat eyes.
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Received: 22 October 2021
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| Fund:National Natural Science Foundation of China (31301070); Zhejiang Provincial Natural
Science Foundation of China (Y2100924); Project of Wenzhou Science & Technology Bureau (Y2008002) |
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Corresponding Authors:
Juxiu Ye, State Key Laboratory of Ophthalmology and Visual Science, Wenzhou
Medical Universtiy, Wenzhou 325027, China (Email: yejuxiu@mail.eye.ac.cn)
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