Objective To investigate the effect of corneal crosslinking guided by riboflavin ultravilet rays on flap adhesion and healing in laser in situ keratomileusis (LASIK) in the rabbit.Methods LASIK flaps were created in 22 rabbit corneas in this experimental study.The right eyes were the experimental eyes while the left eyes were the control eyes.The edema and inflammation reaction of the corneas were observed with slit lamp at different time points (1 day,3 days,7 days after surgery).Histopathological changes,including inflammatory cell infiltration and keratocyte distribution in the corneas,were examined at different time points (1 week,1 month,3 months after surgery).One month after surgery,the adhesion of the corneal flap and corneal stroma was detected by a tensile resistance experiment and the diameters of collagen fibers in each group were compared by electron microscope section of 5 rabbit corneas.The data were analyzed by an independent samples t test and a paired t test.Results Yellow-green riboflavin could be found in the anterior chamber after 20 min,which suggested that riboflavin could penetrate the corneal stroma.There was no difference in corneal edema between the experimental and control groups.And the corneal edema had almost disappeared 3 days after LASIK.When compared to the control group,pathology showed that there was no further infiltration of inflammatory cells in the experimental eyes.There was a "no cell zone" in both eyes,and there was no difference between them.At different tension lengths,the adhesion of the corneal flap and the corneal stroma in the experimental eyes was stronger than in the control eyes after 1 month (P＜0.01).In electron microscope sections,mean collagen fiber diameter of the experimental eyes was bigger than in the control eyes (t=7.94,P＜0.01).Conclusion Animal experiments involving photodynamic corneal crosslinking gruided by riboflavin-ultravilet rays can promote the adhesion and healing of flaps after laser in situ keratomileusis.