Objective To study the effect of pictorial and disparity depth cues on accommodative response,to analyze the effect of cue conflict on accommodative variability and response in the human visual system.Methods Experimental study.Thirty young adults with normal stereoscopic function participated in this study.Accommodative responses were continuously measured with a Grand Seiko WAM-5500 infrared auto-refractor under binocular viewing conditions.The targets were presented at a viewing distance of 50 cm on a stereoscopic display that included different depth cues:①no cue,②pictorial depth cue only,③disparity depth cue only,④disparity±pictorial depth cue Data were analyzed using a repeated measures analysis of variance.Results The accommodative responses to the 4 targets were 1.35±0.24 D,1.42±0.27 D,1.40±0.27 D and1.36±0.25 D,There were statistical different responses to the 4 targets (P＜0.05).In the same viewing environment,a strmulus with either a pictorial depth cue only elicited a higher accommodative response than a stimulus with no cue (P＜0.05),disparity depth cue only made higher accommodative response than with nothing cue stimulus (P＜0.05).However,there was no significant difference (P＞0.05) between the accommodative response to the disparity+pictorial depth cues stimulus and a stimulus with no cue.Conclusion Both disparity depth cue and pictorial depth cues increase accommodation,however,the effect of the summation of two depth cues on accommodative response is not a simple combined effect but a complex one.Compared with two-dimensional pictures,accommodative microfluctuations are steadier while viewing stereoscopic pictures using multiple cues in a short time interval.This may be related to focusing attention and positive adaptation using colorful 3D pictures in a short time interval.