白介素1β在干眼患者眼表的表达

doi:10.3760/cma.j.issn.1674-845X.2014.04.009

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Abstract

Objective To study the expression of IL-1 beta on the ocular surface of dry eye patients and to study its correlation with symptoms and signs of dry eye； to investigate IL-1 beta′s effect on dry eye pathogenesis. Methods Clinical trials. In this case-control study， 30 dry eye patients （60 eyes） and 15 age- and gender-matched control subjects （30 eyes） without symptoms or signs of dry eye were selected from patients who had been treated in our hospital from September 2012 to February 2013. All subjects underwent full ophthalmologic examinations （including OSDI questionnaire， Schirmer′s test （SIT）， tear break-up time （BUT）， corneal fluorescence staining and conjunctival lissamine green staining）. Impression cytology was used to collect conjunctiva epithelial cells from dry eye patients and controls， then these samples were stained by IHC reaction to detect the expression of IL-1 beta by real-time PCR. Results The average OSDI questionnaire scores for the case group and controls were 24.1±2.2 and 14.3±1.3. SIT scores were 4.13±1.68 mm and 10.53±0.74 mm. BUT times were 4.17±1.10 s and 8.80±1.21 s. Corneal fluorescence staining results were 4.3±1.5 and 0.6±0.5. Conjunctival lissamine green staining results were 5.7±2.0 and 1.9±1.4. There were significant differences between dry eye patients and control subjects （t=22.23， -19.88， -18.20， 12.85， 9.62， P<0.05）. The average expression of IL-1 beta in the case group and controls was 0.65±0.37 and 0.22±0.06. There were significant differences between dry eye patients and control subjects （t=6.31， P<0.05）. The expression of IL-1 beta was positively correlated with scores on the OSDI questionnaire （r=0.81， P<0.05）， corneal fluorescence staining （r=0.58， P<0.05）， and conjunctival lissamine green staining （r=0.48， P<0.05）， and was negatively correlated with BUT （r=-0.45， P<0.05） and SIT （r=-0.43， P<0.05）. With IHC staining， brown particles could be seen in the conjunctiva cells in patients with dry eye， but not in the controls. Conclusion IL-1β as an inflammatory mediator is involved not only in dry eye disease， but is also associated with the severity of dry eye and damage on the ocular surface.

Key words： Interleukin-1 beta Xerophthalmia Impression cytology Real-time polymerase chain reaction

Received: 25 October 2013

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	Articles by authors
	Gao Ya
	Li Bing
	Chen Yanxia
	Chang Jing
	Zheng Xiaofen

Cite this article:

Gao Ya,Li Bing,Chen Yanxia等. A study of the expression of IL-1 beta on the ocular surface in dry eye patients[J]. Chinese Journal of Optometry Ophthalmology and Visual Science, 2014, 16(4): 228-232.

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https://www.cjoovs.com/EN/10.3760/cma.j.issn.1674-845X.2014.04.009 OR https://www.cjoovs.com/EN/Y2014/V16/I4/228

[1]	The definition and classification of dry eye disease： report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop （2007）[J]. Ocul Surf，2007，5：75-92.
[2]	刘洪婷，刘磊. 准分子激光屈光性角膜手术后干眼症的研究进展[J]. 眼视光学杂志，2004，6：145-148.
[3]	Jones DT ， Monroy D， Ji Ｚ， et al. Alterations of ocular surface gene expression in Sjogren′s syndrome[J]. Adv Exp Med Biol，1998，438：533-536.
[4]	Pflugeflder SC， Jones D， Ji Z， et al. Altered cytokine balance in zhe tear fluid and conjunetiva of patients with Sjogren′s syndrome keratoeonjunetivitis sicca[J]. Curr Eye Res，1999，19：201-211.
[5]	Inarello CA. Biologic basis for interleukin-1 in desease[J]. Blood，1996，15，2095-2147.
[6]	Solomon A， Dursun D， Liu Z， et al. Pro-and anti-inflammation forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease[J]. Invest Ophthalmol Vis Sci，2001，42：2283-2292.
[7]	Niederkorn JY， Stern ME， Pflugfelder SC， et al. Desiccating stress induces T cell-mediated Sjögren′s syndrome-like lacrimal keratoconjunctivitis[J]. J Immunol，2006，176：3950-3957.
[8]	El Annan J， Chauhan SK， Ecoiffier T， et al. Characterization of effector T cells in dry eye disease[J]. Invest Ophthalmol Vis Sci，2009，50：3802-3807.
[9]	De Paiva CS， Chotikavanich S， Pangelinan SB， et al. IL-17 disrupts corneal barrier following desiccating stress[J]. Mucosal Immunol，2009，2：243-253.
[10]	Zheng X， de Paiva CS， Li DQ， et al. Desiccating stress promotion of Th17 differentiation by ocular surface tissues through a dendritic cell mediated pathway[J]. Invest Ophthalmol Vis Sci，2010，51：3083-3091.
[11]	Chauhan SK， El Annan J， Dana R， et al. Autoimmunity in dry eye is due to resistence of Th 17 to Treg suppression[J]. J Immuol，2009，182：1247-1252.
[12]	Dinarello CA. Interleukin-1 beta[J]. Crit Care Med，2005，33：460-462.
[13]	Limb GA， Little BC， Meager A， et al. Cytokines in proliferative vitreoretinopathy[J]. Eye，1991，5：686-693.
[14]	冯学峰，惠延年，王雨生. IL-1β与人视网膜色素上皮细胞胶原合成的作用[J]. 第四军医大学学报，1999，20：656-658.
[15]	Solomon A， Dursun D， Lin Z， et al. Pro- and anti-inflammatory forms of interleukin-1 in the tear fluid and conjuctiva of patients with dry eye disease[J]. Invest Ophthalmol Vis Sci，2001，42：2283-2292.
[16]	李军，李丹，范春霞，等.干眼症患者结膜上皮细胞和泪液中TNF-α、IL-1β的表达及意义[J]. 细胞与分子免疫学杂志，2010， 26：1128-1129.
[17]	刘祖国，杨文照. 干眼症的发病机制[J]. 眼科，2005：342-345.
[18]	Gorelik L， Flavell RA. Abrogation of TGF-β signaling in T cells leads to spontaneous T cell differentiation and autoimmune disease[J]. Immunity，2000，12：171-181.