Objective  To demonstrate the feasibility of visualizing the ciliary muscle using 1 310 nm wavelength spectral domain optical coherence tomography （OCT）. Methods  Experimental study. Three normal prepresbyopic subjects were recruited. 1 310 nm spectral domain OCT was used to image the temporal ciliary muscles of the left eyes in relaxed-accommodative and maximum-accommodative states. Custom software was used to correct and process OCT images. The thicknesses of the ciliary muscle 1 mm， 2 mm and 3 mm posterior to the scleral spur （CMT1， CMT2， CMT3） and the maximum thickness of the ciliary muscle （CMTM） were measured. The area of the ciliary muscle anterior to the CMT1 line served as CMA1. Similarly， the areas of the ciliary muscle between the CMT1 and CMT2 lines and between the CMT2 and CMT3 lines were defined as CMA2 and CMA3， respectively. Results  The high-resolution images of ciliary muscles were clearly captured in two different accommodative states. The boundaries of the cilairy muscle were easily visualized， and the custom software was feasible for the correction and analysis of the images. CMTM， CMT1 and CMT2 in the relaxed-accommodative state were thinner than in the maximum-accommodative state. In contrast， CMT3 in the relaxed-accommodative state was thicker than in the maximum-accommodative state. Similarly， CMA1 and CMA2， but not CMA3， were decreased in the relaxed-accommodative state as compared to the maximum-accommodative state. Conclusion  1 310 nm spectral domain OCT is a potentially promising technique for non-contact， non-invasive， real-time and high-resolution imaging of the ciliary muscle in human eyes.