Objective To compare the morphological and electrophysiological properties of human and mouse retinal bipolar cells. Methods This was an experimental study. Immunofluorescence staining on cryosections of the retina was used to study the expression patterns of protein kinase C alpha (PKC-α) in human and mouse retinas. Retinal rod bipolar cells were labeled with PKC-α antibody. Using voltage clamp recording, glutamate currents from retinal ON bipolar cells and OFF bipolar cells were induced by applying 40 µmol/L LY341495 and 100 µmol/L AMPA, respectively. Data were analyzed using nonparametric tests (Mann-Whitney test). Results Retinal rod bipolar cells were similar in mice and humans, with slight differences in the PKC-α expression pattern. Rod bipolar cell bodies were located in the human and mouse INL (inner nuclear layer) with PKC-α staining. Using whole-cell recording, we found that there was a significant difference in LY341495-current kinetics in the human and mouse retinal ON bipolar cells (U=0.00, P＜0.01) but not in the kinetics of the AMPA-induced current, including amplitude (humans: 143.0±2.1 pA; mice: 136.3±2.2 pA), time to peak(humans: 1.91±0.35 ms; mice: 1.28±0.52 ms) and time to recovery (humans: 220.5±10.8 ms; mice: 168.1±29.3 ms) in the OFF bipolar cells. Conclusion Rod bipolar cells are expressed in both mouse and human retinas in a similar pattern with only a slight difference. Our results show the different simulated light response properties between human and mouse retinal ON bipolar cells.
罗雪,沈雨濛,江梦南,刘诗亮,胡单萍,沈吟. 人和小鼠视网膜双极细胞的形态及功能比较[J]. 中华眼视光学与视觉科学杂志, 2016, 18(1): 14-18.
Luo Xue,Shen Yumeng,Jiang Mengnan,Liu Shiliang,Hu Danping,Shen Yin. Function and morphological characteristics of bipolar cells in human and mouse retinas. Chinese Journal of Optometry Ophthalmology and Visual science, 2016, 18(1): 14-18. DOI: DOI:10.3760/cma.j.issn.1674-845X.2016.01.004
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