Abstract：Metagenomic next-generation sequencing (mNGS), which enables unbiased analysis of the total microbial and host genes in patients, is increasingly applied to diagnose rare, complex, and severe infections. Moreover, mNGS has broad application prospects in pathogen subtype classification, drug resistance, virulence analysis. Ocular opportunistic infection by immunodeficiency in human immunodeficiency virus (HIV) patients can severely damage the structure and function of eye tissues which is a major cause of visual impairment and blindness in these patients. The pathogenic microorganisms include bacteria, viruses, fungi, spirochetes, and parasites. The ocular opportunistic infections in HIV patients are challenging to diagnose because of the variable clinical symptoms and even mixed infections, meanwhile traditional pathogen detection methods are restricted to apply due to the limited volume of the eyeball and low cell count of intraocular fuids for sampling, and poor specifcity. Compared to traditional detections, metagenomic next-generation sequencing can accurately identify all pathogens causing eye infections in HIV patients with small samples and provide analysis of pathogen virulence and drug resistance. This review will categorize the application of mNGS technology in unbiased microbial detection and analysis of concurrent opportunistic eye infections in HIV patients.
王罗梓怡, 姜靖, 王志良. 深度测序技术在HIV感染并发眼内机会感染临床中的应用[J]. 中华眼视光学与视觉科学杂志, 2023, 25(7): 555-559.
Luoziyi Wang, Jing Jiang, Zhiliang Wang. Study of the Clinical Application of Next-Generation Sequencing in HIV Ocular Complications. Chinese Journal of Optometry Ophthalmology and Visual science, 2023, 25(7): 555-559. DOI: 10.3760/cma.j.cn115909-20221129-00469
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