Abstract：Objective: To investigate the pathogenic microbial spectrum and seasonal distribution characteristics of infectious keratitis in southern China. Methods: In this retrospective case series study, all results of corneal microbiological examinations of patients with infectious keratitis, from July 1, 2013 to December 31, 2017, at Zhongshan Ophthalmic Center, Sun Yat-sen University, were reviewed and analyzed. The scraped corneal specimens of patients with keratitis were subjected to an examination of bacteria and fungi in the hospital's clinical laboratory. A chi square test was used for statistical analysis. Results: Of 3 914 samples, 2 468(63.1%) were from males and 1 446(36.9%) were from females, with an average age of 49.5±16.8 years. The microbial testing was positive in 1 673 cases (42.7%). Among them, 749(44.8%) were positive for bacteria, 849 (50.7%) were positive for fungi, and 75(4.5%) were co-infections with both fungi and bacteria. The most common type of bacteria isolated was Staphylococcus epidermidis (20.1%), followed by Pseudomonas aeruginosa (7.5%). The most common type of fungi was the Fusarium species (25.5%), followed by the Aspergillus species (10.3%). The prevalence of bacteria was significantly higher in May to August than in September to February of the following year (P<0.05), and the prevalence of fungi was significantly lower in May to June than in September to February of the following year (P<0.05). Conclusions: Fungi infection is slightly higher than bacterial infection in infectious keratitis in southern China. There are seasonal variations in bacterial and fungal infections.
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