角膜后基质与后弹力层交界面的超微结构

doi:10.3760/cma.j.issn.1674-845X.2019.02.004

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摘要目的研究不同年龄段角膜后基质与后弹力层交界面的超微结构。方法实验研究。选取不同年龄来源的新鲜供体角膜植片14枚,分为婴幼儿组（3枚）、成年组（5枚）、老年组（6枚）。将供体植片固定于钻台上,台盼蓝溶液染色,8.0 mm环钻轻压,用无齿显微镊将后弹力层和角膜内皮层撕离,暴露一层光滑的界面（CⅠ）;显微镊继续撕除一半光滑的界面,暴露出相对粗糙的界面（CⅡ）。取2个界面组织行扫描电子显微镜观察、苏木精-伊红（HE）染色及胶原纤维免疫荧光染色。结果光学显微镜下可见3组供体角膜CⅠ界面均光滑,CⅡ界面均粗糙。扫描电子显微镜下发现3组供体角膜CⅠ界面位于后弹力层之前,为一层光滑的后弹力层前膜,CⅡ界面可见粗大呈束状的后基质层胶原纤维。HE染色可见角膜后弹力层附着于基质,撕除完整后弹力层,暴露光滑的后弹力层前膜,3组供体角膜未见明显差别。3组供体角膜胶原纤维免疫荧光染色显示后弹力层前膜存在Ⅰ、Ⅲ、Ⅵ型胶原纤维,后弹力层含Ⅳ型胶原纤维。结论不同年龄段角膜后弹力层与后基质层之间均存在一层薄且光滑的后弹力层前膜,主要由Ⅰ、Ⅲ、Ⅵ型胶原纤维组成。后弹力层前膜与后弹力层连接疏松,与后基质连接紧密。

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	林威
	朱奕睿
	陈蔚

关键词 ：后基质, 后弹力层, 交界面, 后弹力层前膜, 超微结构

Abstract：Objective: To reveal the ultrastructural characteristics of the interface between Descemet's membrane (DM) and posterior stroma at different ages. Methods: In this experimental study, corneal buttons (n=14) were divided into three groups, infants (n=3, 0-6 years), adults (n=5, 21-48 years), and elderly (n=6, 63-87 years). Each corneal button was mounted on a suction block, and the endothelium was gently marked with an 8.0 mm trephine and then stained with trypan blue. Smooth forceps were used to strip off the DM from the mark at the central edge, exposing the first cleavage plane (CⅠ). Then half of the CⅠ was scraped to expose the second cleavage plane (CⅡ). Samples were observed by scanning electron microscopy and by hematoxylin-eosin (HE) staining and immunofluorescence staining for collagen types Ⅰ, Ⅲ, Ⅳ, Ⅴ and Ⅵ. Results: The CⅠ of all 14 donor corneas were smooth under light microscopy, while CⅡ were relatively rough. Scanning electron microscopy showed that the CⅠ (pre-DM membrane) was thin and smooth, while the CⅡ (posterior stroma) was composed of relatively rough stromal fiber bundles. HE staining showed that where DM was attached to the stroma, the smooth pre-DM membrane was exposed after stripping DM. Immunofluorescence microscopy showed that the pre-DM membrane was strongly positive for collagen type Ⅰ and Ⅲ. Collagen type Ⅵ was topical positive in the pre-DM membrane. There was no significant difference in three groups. Conclusions: A thin, smooth pre-DM membrane consisting of collagen type Ⅰ, Ⅲ, and Ⅵ was present at the DM-stromal interface in different ages. The pre-DM membrane was tightly attached to the stroma, but only loosely to DM.

Key words： posterior stroma Descemet's membrane interface pre-Descemet's membrane ultrastructure

收稿日期: 2017-01-04

通讯作者: 陈蔚（ORCID：0000-0001-5375-1199）,Email：chenweimd@hotmail.com

作者简介: 林威（ORCID：0000-0002-3492-5488）,Email：linweiysg@163.com

引用本文:

林威, 朱奕睿, 陈蔚. 角膜后基质与后弹力层交界面的超微结构[J]. 中华眼视光学与视觉科学杂志, 2019, 21(2): 97-103. Wei Lin, Yirui Zhu, Wei Chen. Ultrastructure of the Interface between Descemet's Membrane and the Posterior Stroma. Chinese Journal of Optometry Ophthalmology and Visual science, 2019, 21(2): 97-103. DOI: 10.3760/cma.j.issn.1674-845X.2019.02.004

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https://www.cjoovs.com/CN/10.3760/cma.j.issn.1674-845X.2019.02.004 或 https://www.cjoovs.com/CN/Y2019/V21/I2/97

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