Objective To evaluate new logMAR visual acuity chart′s test-retest reliability and concordance with “C” logarithm visual acuity chart. Methods This was a cross-sectional study. Measurements using two versions of each chart design were obtained on the right eye of 48 high school students. The test-retest reliability was analyzed by intraclass correlation coefficient （ICC） and Cronbach′s Alpha coefficients， and Bland-Altman plots were used to compare the agreement between the two charts. Results The Cronbach′s Alpha coefficients of “C” logarithm visual acuity chart was more than 0.8， and the ICC was near 0.9. For the new logMAR visual acuity chart， the Cronbach′s Alpha coefficients was near 0.8， and the ICC was more than 0.75. The Bland-Altman plots displayed relatively good agreement between the two charts. The 95％ limits of agreement were 0.173 to -0.133 logMAR and 0.198 to -0.116 logMAR for the test and retest measurements respectively. Conclusion In healthy eyes， the new logMAR visual acuity chart has high test-retest reliability and good concordance with Landolt C logarithmical visual acuity chart.