Objective To conduct research on the relationship between LCD visual acuity chart display accuracy and LCD display pixel size. Methods Standard control and measurement methods were used to assess the LCD-related parameters of the new standard logarithmic visual acuity chart relative to the national standard (GB11533-2011). A comparative study of optotype sizes versus permissible errors in the LCD chart was done. Results The LCD visual acuity chart was fully compliant for brightness, width, whiteness, and lighting requirements (GB11533-2011). For liquid crystal display precision, a 0.27 mm pixel size can accurately test distance visual acuity ≤5.0; a 0.1245 mm pixel size can accurately test distance visual acuity ≤5.2; a 0.077 mm pixel size can accurately test distance visual acuity ≤5.3. Conclusion Select the appropriate distance and LCD display pixel size to more accurately test visual acuity.