Outcome comparison of fresh and glycerin-cryopreserved corneal tissue in treating purulent keratitis using deep anterior lamellar keratoplasty " />
新鲜与甘油冷冻保存角膜供体应用于深板层角膜移植治疗化脓性角膜炎
Objective To compare the outcomes of using fresh and glycerin-cryopreserved corneal tissue in treating purulent keratitis with deep anterior lamellar keratoplasty. Methods This was a retrospective case series study. Thirty-two patients （32 eyes） with purulent keratitis successfully underwent deep anterior lamellar keratoplasty （DALK） in Shandong Eye Hospital from January 2011 to August 2012. Based on the method for preserving corneal tissue， all patients were divided into a fresh corneal tissue （FCT） group （13 cases） and a glycerin-cryopreserved corneal tissue （GCCT） group （19 cases）. Data on donor， complications， graft status and visual outcome were collected. An independent samples t test analysis was used to analyze the data. Results The amount of time for the epithelium graft to heal in the FCT group and GCCT group were 3.8±2.2 d and 7.3±2.7 d （t=-3.764， P<0.05）. The amount of time for the graft edema to disappear in the FCT group and GCCT group were 5.7±2.6 d and 10.4±3.0 d （t=-4.348， P<0.01）. In the follow-up phase， 3 cases of graft epithelium defects occurred in the GCCT group and were healed with symptomatic treatment. After the surgery， the best corrected visual acuity （BCVA） of 23 patients （excluding cataract and congenital amblyopia） improved to 20/63-20/25， the mean BCVAs of the FCT and GCCT groups were 0.25±0.12 logMAR and 0.28±0.17 logMAR， respectively （t=-1.428， P>0.05）. Conclusion When glycerin-cryopreserved corneal tissue is used to treat purulent keratitis in DALK， the amount of time for the epithelium to heal and corneal edema to disappear took longer than with fresh corneal tissue. If potential or actual complications in GCCT group were treated in a timely and reasonable manner， similar outcomes would be obtained as for the FCT group.
宋鹏,隋文婕,丁刚,贾艳妮,李素霞,高华. 新鲜与甘油冷冻保存角膜供体应用于深板层角膜移植治疗化脓性角膜炎[J]. 中华眼视光学与视觉科学杂志, 2013, 15(10): 612-615.
SONG Peng,SUI Wen-jie,DING Gang,JIA Yan-ni,LI Su-xia,GAO Hua. Outcome comparison of fresh and glycerin-cryopreserved corneal tissue in treating purulent keratitis using deep anterior lamellar keratoplasty . Chinese Journal of Optometry Ophthalmology and Visual Science, 2013, 15(10): 612-615. DOI: 10.3760/cma.j.issn.1674-845X.2013.10.008
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