Abstract：Corneal collagen cross-linking (CXL) is a photochemical method which uses light to irradiate the cornea infiltrated by photosensitizers, leading to covalent bond formation (i.e., crosslinks) within and between macromolecules, potentially including collagen and other matrix proteins. This method stiffens the cornea and maintains its biomechanical stability. The traditional ribo?avin ultraviolet-A-induced corneal collagen cross-linking (UVX) uses ribo?avin as a photosensitizer, and then the cornea is irradiated by 370 nm ultraviolet light. Although UVX can certainly maintain corneal biomechanical stability, its side effects and limitations cannot be ignored. As a novel CXL technology, collagen cross-linking with rose bengal-green light (RGX)uses rose-bengal as a photosensitizer that is irradiated by 532 nm green light. RGX can be used to treat keratoconus, infectious keratitis and promote corneal wound healing. This paper summarizes its principle and process, safety and effectiveness, as well as its ocular applications and its comparison with UVX.
陈雪阳,高蓉蓉,王勤美,等. 孟加拉玫瑰红-绿光角膜胶原交联术的研究进展[J]. 中华眼视光学与视觉科学杂志, 2021, 23(11): 870-874.
Xueyang Chen, Rongrong Gao, Qinmei Wang,et al. Research Progress on Collagen Cross-Linking with Rose Bengal-Green Light. Chinese Journal of Optometry Ophthalmology and Visual science, 2021, 23(11): 870-874. DOI: 10.3760/cma.j.cn115909-20200313-00088
Rubinfeld RS, Caruso C, Ostacolo C. Corneal Cross-Linking: The science beyond the myths and misconceptions. Cornea, 2019, 38(6): 780-790. DOI: 10.1097/ICO.0000000000001912.
[2]
Cherfan D, Verter EE, Melki S, et al. Collagen cross-linking using rose bengal and green light to increase corneal stiffness. Invest Ophthalmol Vis Sci, 2013, 54(5): 3426-3433. DOI: 10.1167/iovs.12-11509.
[3]
Redmond RW, Kochevar IE. Medical applications of rose bengal- and riboflavin-photosensitized protein crosslinking. Photochem Photobiol, 2019, 95(5): 1097-1115. DOI: 10.1111/ php.13126.
[4]
Wertheimer CM, Elhardt C, Kaminsky SM, et al. Enhancing rose bengal-photosensitized protein crosslinking in the cornea. Invest Ophthalmol Vis Sci, 2019, 60(6): 1845-1852. DOI: 10.1167/iovs.19-26604.
[5]
Bekesi N, Kochevar IE, Marcos S. Corneal biomechanical response following collagen cross-linking with rose bengalgreen light and riboflavin-UVA. Invest Ophthalmol Vis Sci, 2016, 57(3): 992-1001. DOI: 10.1167/iovs.15-18689.
[6]
Gallego-Muñoz P, Ibares-Frías L, Lorenzo E, et al. Corneal wound repair after rose bengal and green light crosslinking: clinical and histologic study. Invest Ophthalmol Vis Sci, 2017,58(9): 3471-3480. DOI: 10.1167/iovs.16-21365.
[7]
Zhu H, Alt C, Webb RH, et al. Corneal crosslinking with rose bengal and green light: efficacy and safety evaluation. Cornea, 2016, 35(9): 1234-1241. DOI: 10.1097/ICO.0000000000000916.
[8]
Wang T, Zhu L, Zhu J, et al. Subacute effects of rose Bengal/ Green light cross linking on rabbit thin corneal stability and safety. Lasers Surg Med, 2018, 50(4): 324-332. DOI: 10.1002/ lsm.22762.
[9]
Wang T, Peng Y, Shen N, et al. Photochemical activation increases the porcine corneal stiffness and resistance to collagenase digestion. Exp Eye Res, 2014, 123: 97-104. DOI: 10.1016/j.exer.2014.04.008.
[10]
Fadlallah A, Zhu H, Arafat S, et al. Corneal Resistance to Keratolysis After Collagen Crosslinking With Rose Bengal and Green Light. Invest Ophthalmol Vis Sci, 2016, 57(15): 6610- 6614. DOI: 10.1167/iovs.15-18764.
[11]
Bekesi N, Gallego-Muñoz P, Ibarés-Frías L, et al. Biomechanical Changes After In Vivo Collagen Cross-Linking With Rose Bengal-Green Light and Riboflavin-UVA. Invest Ophthalmol Vis Sci, 2017, 58(3): 1612-1620. DOI: 10.1167/iovs.17-21475.
[12]
Görner H. Oxygen uptake after electron transfer from amines, amino acids and ascorbic acid to triplet flavins in air-saturated aqueous solution. J Photochem Photobiol B, 2007, 87(2): 73-80. DOI: 10.1016/j.jphotobiol.2007.02.003.
[13]
Alarcon EI, Poblete H, Roh H, et al. Rose Bengal Binding to Collagen and Tissue Photobonding. ACS Omega, 2017, 2(10): 6646-6657. DOI: 10.1021/acsomega.7b00675.
[14]
Lorenzo-Martin E, Gallego-Munoz P, Ibares-Frias L, et al. Rose Bengal and Green Light Versus Riboflavin-UVA Cross-Linking: Corneal Wound Repair Response. Invest Ophthalmol Vis Sci, 2018, 59(12): 4821-4830. DOI: 10.1167/iovs.18-24881.
[15]
Halili F, Arboleda A, Durkee H, et al. Rose Bengal- and Riboflavin-Mediated Photodynamic Therapy to Inhibit Methicillin-Resistant Staphylococcus aureus Keratitis Isolates. Am J Ophthalmol, 2016, 166: 194-202. DOI: 10.1016/ j.ajo.2016.03.014.
[16]
Wollensak G, Iomdina E. Long-term biomechanical properties of rabbit cornea after photodynamic collagen crosslinking. Acta Ophthalmol, 2009, 87(1): 48-51. DOI: 10.1111/j.1755- 3768.2008.01190.x.
[17]
Kling S, Remon L, Pérez-Escudero A, et al. Corneal biomechanical changes after collagen cross-linking from porcine eye inflation experiments. Invest Ophthalmol Vis Sci, 2010, 51(8): 3961-3968. DOI: 10.1167/iovs.09-4536.
O'Brart DP, Patel P, Lascaratos G, et al. Corneal Cross-linking to Halt the Progression of Keratoconus and Corneal Ectasia: Seven-Year Follow-up. Am J Ophthalmol, 2015, 160(6): 1154- 1163. DOI: 10.1016/j.ajo.2015.08.023.
[20]
Proaño CE, Mulroy L, Jones E, et al. Photochemical keratodesmos for bonding corneal incisions. Invest Ophthalmol Vis Sci, 2004,45(7): 2177-2181. DOI: 10.1167/iovs.03-1066.
[21]
Wang T, Zhu L, Peng Y, et al. Photochemical Cross-Linking for Penetrating Corneal Wound Closure in Enucleated Porcine Eyes. Curr Eye Res, 2017, 42(11): 1413-1419. DOI: 10.1080/ 02713683.2017.1344712.
[22]
Verter EE, Gisel TE, Yang P, et al. Light-initiated bonding of amniotic membrane to cornea. Invest Ophthalmol Vis Sci, 2011, 52(13): 9470-9477. DOI: 10.1167/iovs.11-7248.
[23]
Soeken TA, Zhu H, DeMartelaere S, et al. Sealing of Corneal Lacerations Using Photoactivated Rose Bengal Dye and Amniotic Membrane. Cornea, 2018, 37(2): 211-217. DOI: 10.1097/ICO.0000000000001389.
[24]
Seiler TG, Engler M, Beck E, et al. Interface Bonding With Corneal Crosslinking (CXL) After LASIK Ex Vivo. Invest Ophthalmol Vis Sci, 2017, 58(14): 6292-6298. DOI: 10.1167/ iovs.17-22426.
[25]
Dai T, Fuchs BB, Coleman JJ, et al. Concepts and principles of photodynamic therapy as an alternative antifungal discovery platform. Front Microbiol, 2012, 3: 120. DOI: 10.3389/ fmicb.2012.00120.
[26]
Durkee H, Arboleda A, Aguilar MC, et al. Rose bengal photodynamic antimicrobial therapy to inhibit Pseudomonasaeruginosa keratitis isolates. Lasers Med Sci, 2020, 35(4): 861- 866. DOI: 10.1007/s10103-019-02871-9.
[27]
Arboleda A, Miller D, Cabot F, et al. Assessment of rose bengal versus riboflavin photodynamic therapy for inhibition of fungal keratitis isolates. Am J Ophthalmol, 2014, 158(1): 64-70.e2. DOI: 10.1016/j.ajo.2014.04.007.
[28]
Atalay HT, Dogruman-Al F, Sarzhanov F, et al. Effect of Riboflavin/Rose Bengal-Mediated PACK-CXL on Acanthamoeba Trophozoites and Cysts in Vitro. Curr Eye Res, 2018, 43(11): 1322-1325. DOI: 10.1080/02713683.2018.1501074.
[29]
Naranjo A, Pelaez D, Arrieta E, et al. Cellular and molecular assessment of rose bengal photodynamic antimicrobial therapy on keratocytes, corneal endothelium and limbal stem cell niche. Exp Eye Res, 2019, 188: 107808. DOI: 10.1016/ j.exer.2019.107808.
[30]
Amescua G, Arboleda A, Nikpoor N, et al. Rose Bengal Photodynamic Antimicrobial Therapy: A Novel Treatment for Resistant Fusarium Keratitis. Cornea, 2017, 36(9): 1141-1144. DOI: 10.1097/ICO.0000000000001265
浙公网安备 33030202001892号