The Distribution and Expression of 11β-Hydroxysteroid Dehydrogenase
Type 1 in Rat Eyes of Different Ages
Xin'an Chen 1, Yong Lin 2, Juxiu Ye
1School of Basic Medical Science, Wenzhou Medical Universtiy, Wenzhou 325035, China
2State Key Laboratory of Ophthalmology and Visual Science, Wenzhou Medical Universtiy, Wenzhou325027, China
Abstract:Objective: To observe the distribution and expressive changes of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in different age’s rat eyes. Methods: In this experimental research, according to age, SD rats were divided into 6 groups with 6 subjects in each: postnatal days 1-2 (PND1) group, postnatal days 5-6(PND5) group, postnatal days 10-11 (PND10) group, postnatal days 14-15 (PND15) group, postnatal days 20-21 (PND20) group, and 8-9 weeks mature adults rats (M) group. Immunohistochemistry (IHC) assay was used to detect the distribution of 11β-HSD1 protein in the eyes of SD rats at different developmental stages from neonatal, postnatal eyelid opening to mature adult, and the expressions of 11β-HSD1 mRNA and protein levels in the retina or cornea were further verified by real time RT-PCR and Western blot. The data were analyzed by one-way ANOVA or independent sample t-test. Results: 11β-HSD1 protein was widely expressed in such as cornea, iris, retina, choroid, lens, ciliary body of the different age’s rat eyes by IHC staining, and there was more 11β-HSD1 positive cells in neonatal rat eyes. Real time RT-PCR showed that the relative expression of mRNA of 11β-HSD1 peaked at postnatal days 10 in the cornea and decrease gradually after eyelid opening of SD rats (F=8.40, P<0.001); the relative expression of 11β-HSD1 mRNA in the retina of SD rats before eyelid opening was significantly higher than that of eyelid opening rats and adult rats (F=83.50, P<0.001). It was confirmed the relative expression of 11β-HSD1 protein was significantly higher in the retina of neonatal rats than that of eyelid opening rats and mature adult rats by western blot (t=4.76, P=0.009; t=4.24, P=0.013). Conclusion: 11β-HSD1 is widely distributed in SD rat eyes of different ages. The level of 11β-HSD1 expression varies in different age groups. It tends to be higher in eyes of SD rat before eyelid opening. The study suggests that 11β-HSD1 may play an important role in the differentiation, development and maturation of the SD rat eyes.
陈薪安, 林永, 叶菊秀. . 11β-羟基类固醇脱氢酶Ⅰ型在不同年龄段
大鼠眼内的表达与分布[J]. 中华眼视光学与视觉科学杂志, 2022, 24(8): 561-567.
Xin'an Chen 1, Yong Lin 2, Juxiu Ye. The Distribution and Expression of 11β-Hydroxysteroid Dehydrogenase
Type 1 in Rat Eyes of Different Ages. Chinese Journal of Optometry Ophthalmology and Visual science, 2022, 24(8): 561-567. DOI: 10.3760/cma.j.cn115909-20211022-00425