Objective This study aims to determine the changes in the biomechanical properties of the anterior and extreme posterior portions of experimentally induced myopic eyes by examining the mechanical behavior of guinea pig scleral desmocytes,thus finding a new approach to the pathogenesis of myopia and the corresponding therapies.Methods Experimental study.Guinea pigs (2 weeks old) were numbered and assigned into 3 groups (A,B,and C),each with 10 guinea pigs.Concave lens-induced myopic (LIM) animal models were prepared via the out-of-focus method.The other eye in the same guinea pig served as the self-control (SC) group.After modeling groups A,B,and C for 6,15,and 30 days,respectively,the lenses from the guiuea pigs in the experimental group were removed.The scleral fibroblasts in each group were cultured and passaged twice in vitro.The micropipette aspiration technique coupled with a viscoelastic solid model was utilized to investigate the viscoelastic properties of the scleral fibroblasts in normal and group at 30 days were significantly higher than those of the LIM group at 6 and 15 days,as well as those in the SC group (P＜0.05),The extreme posterior portions of the sclera in the LIM group at 6 days after myopic induction did not differ significantly from those of the SC group (P＜0.05).However,the extreme posterior portions of the sclera in the LIM group after 15 days and 30 days were significantly higher than those in the LIM group at 6 days and the SC group (P＜0.05).Conclusion Young's modulus and apparent viscosity of the anterior and extreme posterior portions of the sclera changed by the 15th and 30th days,respectively,after induction.