Objective To analyze the changes of the modulation transfer function (MTF) with or without different mode higher order aberration in normal myopic eyes. Methods Two hundred and fifty-five patients (255 eyes) were included. An aberrometer with Hartmann-Shack sensor was used to measure the monochromatic aberration of eyes. MTF of optical system were calculated at various pupil sizes (3 and 6 mm) respectively and the difference between MTF with whole high order aberrations and MTF without different Zernike mode (C3-3-C33,C40) and different mode of higher-order aberration (S3, S4, S5, S6) with paired-t test. Results The MTF values improved at all spatial frequency [5-60 cycles per degree, (c/d)] without C3-3-C33, C40 respectively both at 3 mm and 6 mm pupil (P＜0.01). As for the effect of each Zernike mode, for a 3 mm pupil, MTF values got the most improvement without C3-1 and C3-3, following with without C31 and C33, and least without C40; for a 6 mm pupil, the improvement of the MTF without C40 was the most, secondly without C3-1 and C3-3,and thirdly without C31 and C33. As for the effects of each order aberrations, for a 3 mm pupil, MTFvalues got significantly improvement without S3 and S4 at all spatial frequency (5-60 c/d) (P＜0.01);for a 6 mm pupil, all MTF values got significantly improvement without S3, S4, S5 and S6 respectively at all spatial frequency (5-60 c/d) (P＜0.01), and without S3 and S4, MTF value increased more than without S5 and S6. Without S3, MTF value increased more than without S4 when the spatial frequency was 20 c/d or lower, and there were no significantly differences between MTF values without S3 and without S4 when the spatial frequency was higher than 20 c/d. MTF value decreased by a constant speed with the spatial frequency increasing at a 3 mm pupil, while at 6 mm pupil, MTF value decreased sharply below 30 c/d, and keep relative stable more than 30 c/d.Conclusion ①The MTF values improve at all spatial frequency (5-60 c/d ) without C3-3-C33 and C40 respectively at different pupil size.