Objectivt To study the value of the application of artificial iris recognition for laser in situ keratomileusis (LASIK). Methods A total of 314 patients with myopic astigmatism were recruited for the study. The patients was divided into 2 groups by whether or not artificial iris recognition was taken during the operation. The patients with artificial iris recognition were group Ⅰ,and the others were group Ⅱ. There were 162 patients (205 eyes) in group Ⅰ, astigmatism range -1.0- -3.0(-1.56±0.79)D, 110 males (141 eyes), 52 females (64 eyes), and mean age (22.6±5.2)years. There were 152 patients (205 eyes) in group Ⅱ, astigmatism range -1.0--3.0(-1.48±0.65)D, 101 males (135 eyes), 51 females (70 eyes), mean age (23.4±5.8)years. Astigmatism and axial direction were measured 6 months postoperatively. The differences between the two techniques were compared based on astigmatism and its axis. Results Postoperative astigmatism in group Ⅰwas (-0.73±0.34)D, and in group Ⅱ was (-0.68±0.53)D. There were no significant differences between the two groups (t=-1.200, P=0.231). Postoperative axial direction in group Ⅰ was (13.25±13.09)° and in group Ⅱ was (17.48±17.93)°. There were significant differences between the two groups (t=2.755, P=0.006). Conclusion Artificial iris recognition can help to correct astigmatism and axial direction in LASIK, and it can be applied to refractive surgery in general.