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| Study on the Protective Mechanism of Qishen Prescription on MAPK Pathway in Hypertonic Induced Dry Eye Model Mice |
| Lei Zhao1, Baoqiang Dong1, Tao Zuo2, Zhuqiang Zhang2, Yanhua Jiang3, Xiande Ma1, Xiaowei Yang1 |
| 1Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China 2Department of Ophthalmology, the Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110034, China 3Department of Ophthalmology, the Fourth People's Hospital of Shenyang, Shenyang 110031, China |
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Abstract Objective: To observe the protective effect of Qishen prescription (QSF) on dry eye (DE) model mice induced by hypertonic fuid. Methods: In this experimental study, in January 2021, 60 BALB/c mice were selected and randomly divided into 6 groups according to the random number table method, which were named as control group, model group, western medicine group, and QSF low-dose, medium-dose and high-dose groups, with 10 mice in each group. Except for the control group, the DE mice model was established with hypertonic saline solution. After establishing the successful model, the model group continued to receive hypertonic saline solution to maintain DE. QSF high-dose, medium-dose and low-dose groups were administered hypertonic saline solution eye drops combined with QSF granules through intragastric administration once a day. Western medicine group was given hypertonic saline combined with 0.3% sodium hyaluronate eye drops. All intervention factors were applied continuously for 14 days. Schirmer I test (S T), breakup time (BUT) and Fluorescent (FL) were measured. Then, the ultrastructure of corneal epithelial cells was observed by transmission electron microscopy. The protein expression levels of JNK1, p-JNK1, p38MAPK, p-p38MAPK, ERK1 and p-ERK1 in corneal tissues were measured by Western Blot. One way ANOVA analysis was used for inter group comparison, and LSD-t test was used for pairwise comparison. Results: Compared with the control group, the S T, BUT and FL was signifcantly different in each group (P<0.01). The protein expression levels of p-JNK1, p-p38, p-ERK1 were signifcantly increased in each group (P<0.01). Compared with the model group, the administration group showed improvement in S T, FL, and BUT (P<0.05), while the p-JNK1 expression in each medicine group and the p-p38 and p-ERK1 in the high and medium dose groups of QSF decreased (P<0.05). Compared with the positive medicine group, the high-dose group of QSF exhibited an increase in S T (P=0.048), while the high and medium dose groups of QSF showed improvements in FL (P=0.004, 0.014), and the high-dose group of QSF showed a decrease in the expression of p-JNK1, p-p38, and p-ERK1 (P=0.017, 0.003, 0.001). Electron microscope showed that the number and regularity of corneal epithelial microvilli in each treatment group of QSF were better than those in the model group and western medicine group. Conclusions: QSF can not only effectively improve S T, BUT, FL, corneal epithelial cell microvilli morphology of hypertonic-induced dry eye in mice, but also reduce the phosphorylation of JNK1, p38MAPK, ERK1.
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Received: 07 March 2023
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| Fund:National Natural Science Foundation of China (82104714) |
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Corresponding Authors:
Baoqiang Dong, Email: Peterbaoqiang@163.com
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