Objective  To study the relationship between the higher-order aberrations and low-， medium-， and high-contrast visual acuity in normal human eyes with excellent eyesight. Methods  A descriptive study was conducted and normal human eyes with excellent eyesight were selected for this test. With background brightness set at 260 lumens， visual acuities were measured at 4 contrast levels of 100%， 25%， 10% and 5%. At the same time， wavefront aberrations were measured with pupil diameters at 3.11 mm and 5.96 mm. The correlation between contrast visual acuity and wavefront aberration under different pupil sizes was evaluated， and fitted regression equations were determined by using linear regression analysis. Results  Visual acuity varied more significantly under low contrast levels than under high contrast. When pupil diameter was set at 3.11 mm， there was a linear correlation between the root mean square value （RMS） of higher-order aberrations and contrast visual acuity （mini coefficient of determination R2=0.1129）. Conclusion  Wavefront aberrations impact contrast visual acuity at all contrast levels.