Objective To assess the compliance in glaucoma patients with topical medication and to explore the influential factors of it, in order to design better therapeutic scheme for patients.Methods In this investigative survey of topical medication compliance in glaucoma patients, 120out-patients were selected and a self-designed questionnaire was used. According to the standard recommended by Morisky of evaluating the compliance in glaucoma patients, all the data obtained were analyzed by the SAS 9.1.3 software using a logistic regression analysis. Results Glaucoma patients with topical medication compliance account for 63.9％ of all the patients investigated.Patients' age, incoming level, frequency and method of medication and doctors's notify were correlated with the topical medication compliance in glaucoma patients (x2=18.752, 17.783, 13.530, 9.022,14.831, P＜0.05). Conclusion There is a good topical medication compliance in these glaucoma patients investigated. Patients over 60 years old, over 600 yuan monthly incomings, using eyedrop 3times in a day, correct method and notified by doctors have good topical medication compliance.Simple and reasonable therapeutic scheme, more education of knowledge regard to glaucoma, can improve the topical medication compliance in glaucoma patients.