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The Use of Scanning Electron Microscopy to Evaluate the Effectiveness of Soft Contact Lens Care Solutions in Removing Proteins from Soft Contact Lenses |
Ping Su,Dongqing Lin |
Chalice Solution Pte. Ltd., Danyang 212300, China
Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China |
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Abstract Objective: To evaluate the ability of scanning electron microscopy (SEM) and image computer statistical analysis (ICSA) to quantitatively assess the effectiveness of protein removal from soft contact lenses by soft contact lens care solutions. Methods: Soft contact lenses were soaked in artificial tears for 65 hours to simulate protein adsorption. Two contact lens care solutions and phosphate buffered saline(PBS) were then used separately to rub, rinse, and soak the lenses for 6 hours. After fixation with glutaraldehyde, the lenses were dried. The morphology and quantity of protein on the surface of the lenses were inspected by SEM and ICSA. The cleansing rate was expressed as a mass fraction, i.e., a percentage of before and after treatment with the care solution. Single factor analysis of variance was used to analyze the protein clearance rate of lens treated with different treatments by Tukey multiple comparison method.Results: The contact lens care solution had a significant cleaning effect on the surface protein of the lenses.For PBS and the two kinds of contact lens care solutions from different manufacturers, the protein clearance rates of the lenses were 89.58%, 98.18% and 98.38% at 100× magnification and 89.76%, 98.42% and 98.27% at 1 500× magnification. Conclusion: SEM combined with ICSA can effectively evaluate the protein cleaning efficacy of contact lens care solutions. Contact lens care solutions have a significant cleaning effect on the surface protein of the lenses.
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Received: 22 September 2016
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Corresponding Authors:
Dongqing Lin, Chalice Solution Pte.Ltd., Danyang 212300, China; Department ofBiomedical Engineering, Jinan University, Guangzhou 510632, China (Email: conmasterlin@aliyun.com)
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|
|
|
[2] |
谢培英. 软性接触镜护理液的创新发展. 中国眼镜科技杂志,2012(3): 148-150.
|
[1] |
Eltis M. Contact-lens-related microbial keratitis: case report and review. J Optom, 2011, 4(4): 122-127.
|
[3] |
李若慧, 马榴强. 角膜接触镜材料的应用和研究进展. 化工新型材料, 2009, 37(3): 15-17.
|
[2] |
谢培英. 软性接触镜护理液的创新发展. 中国眼镜科技杂志,2012(3): 148-150.
|
[4] |
Mann A, Tighe B. Contact lens interactions with the tear film.Experiment Eye Res, 2013, 117: 88-98.
|
[3] |
李若慧, 马榴强. 角膜接触镜材料的应用和研究进展. 化工新型材料, 2009, 37(3): 15-17.
|
[5] |
Subbaraman LN, Glasier MA, Varikooty J, et al. Protein deposition and clinical symptoms in daily wear of etafilcon lenses.Optom Vis Sci, 2012, 89(10): 1450-1459.
|
[4] |
Mann A, Tighe B. Contact lens interactions with the tear film.Experiment Eye Res, 2013, 117: 88-98.
|
[6] |
Omali NB, Zhao Z, Zhu H, et al. Quantification of individual proteins in silicone hydrogel contact lens deposits. Mol Vis,2013, 19(4): 390-399.
|
[5] |
Subbaraman LN, Glasier MA, Varikooty J, et al. Protein deposition and clinical symptoms in daily wear of etafilcon lenses.Optom Vis Sci, 2012, 89(10): 1450-1459.
|
[7] |
张迎书. 大学生人群中巨乳头性结膜炎发病情况的调查分析.现代实用医学, 2010, 22(8): 898-899.
|
[6] |
Omali NB, Zhao Z, Zhu H, et al. Quantification of individual proteins in silicone hydrogel contact lens deposits. Mol Vis,2013, 19(4): 390-399.
|
[8] |
Tripathi RC, Tripathi BJ, Ruben M. The pathology of soft contact lens spoilage. Ophthalmology, 1980, 87(5): 365-380.
|
[7] |
张迎书. 大学生人群中巨乳头性结膜炎发病情况的调查分析.现代实用医学, 2010, 22(8): 898-899.
|
[9] |
林冬青. 角膜接触镜的日常护理(上). 中国眼镜科技杂志,2006(9): 113-116.
|
[8] |
Tripathi RC, Tripathi BJ, Ruben M. The pathology of soft contact lens spoilage. Ophthalmology, 1980, 87(5): 365-380.
|
[10] |
林冬青. 角膜接触镜的日常护理(下). 中国眼镜科技杂志,2006(11): 109-111.
|
[9] |
林冬青. 角膜接触镜的日常护理(上). 中国眼镜科技杂志,2006(9): 113-116.
|
[11] |
文燕, 贾晓航, 何涛. 评价接触镜除蛋白护理液去蛋白的试验方法. 中国医疗器械杂志, 2006, 30(2): 131-133.
|
[10] |
林冬青. 角膜接触镜的日常护理(下). 中国眼镜科技杂志,2006(11): 109-111.
|
[12] |
Cerulli L, Pocobelli A, Ricci F, et al. Two methods of examining protein deposits on hydrophilic contact lenses. Eye contact lens,1992, 18(2): 101-104.
|
[11] |
文燕, 贾晓航, 何涛. 评价接触镜除蛋白护理液去蛋白的试验方法. 中国医疗器械杂志, 2006, 30(2): 131-133.
|
[13] |
Liu Y, Xie P. Quantitative assay of protein deposits on hydrophilic contact lenses treated with renu® and complete® solutions.Int Contact Lens Clin, 1999, 26(1): 15-19.
|
[12] |
Cerulli L, Pocobelli A, Ricci F, et al. Two methods of examining protein deposits on hydrophilic contact lenses. Eye contact lens,1992, 18(2): 101-104.
|
[14] |
李长兵, 孙复钱, 栾洁. 两种不同材料角膜接触镜的蛋白质沉淀分析. 实用临床医药杂志, 2009, 13(4): 108-109.
|
[13] |
Liu Y, Xie P. Quantitative assay of protein deposits on hydrophilic contact lenses treated with renu® and complete® solutions.Int Contact Lens Clin, 1999, 26(1): 15-19.
|
[15] |
Omali NB, Zhao Z, Zhong L, et al. Quantification of protein deposits on silicone hydrogel materials using stable-isotopic labeling and multiple reaction monitoring. Biofouling, 2012, 28(7): 697-709.
|
[14] |
李长兵, 孙复钱, 栾洁. 两种不同材料角膜接触镜的蛋白质沉淀分析. 实用临床医药杂志, 2009, 13(4): 108-109.
|
[16] |
Xin X, Gong T, Hong Y, et al. The influence of long-term corneal contact lens wearing on the stability and quality of tear film in
|
[15] |
Omali NB, Zhao Z, Zhong L, et al. Quantification of protein deposits on silicone hydrogel materials using stable-isotopic labeling and multiple reaction monitoring. Biofouling, 2012, 28(7): 697-709.
|
|
Qinghai. Int J Clin Exp Med, 2016, 9(5): 8416-8420.
|
[16] |
Xin X, Gong T, Hong Y, et al. The influence of long-term corneal contact lens wearing on the stability and quality of tear film in
|
[17] |
Heiler DJ, Gambacorta-Hoffman S, Groemminger SF, et al.The concentric distribution of protein on patient-worn hydrogel
|
|
Qinghai. Int J Clin Exp Med, 2016, 9(5): 8416-8420.
|
|
lenses. Eye contact lens, 1991, 17(4): 249-251.
|
[17] |
Heiler DJ, Gambacorta-Hoffman S, Groemminger SF, et al.The concentric distribution of protein on patient-worn hydrogel
|
[18] |
刘毅, 谢培英, 瞿佳, 等. 生理盐水与护理液对亲水性隐形眼镜蛋白质沉淀洗脱效应的比较. 眼视光学杂志, 2001, 3(3):160-161.
|
|
lenses. Eye contact lens, 1991, 17(4): 249-251.
|
[19] |
Chacon D, Hsieh Y-L, Kurth MJ, et al. Swelling and protein absorption/desorption of thermo-sensitive lactitol-based
|
[18] |
刘毅, 谢培英, 瞿佳, 等. 生理盐水与护理液对亲水性隐形眼镜蛋白质沉淀洗脱效应的比较. 眼视光学杂志, 2001, 3(3):160-161.
|
|
polyether polyol. Polymer, 2000, 41(23): 8257-8262.
|
[20] |
Luensmann D, Jones L. Protein deposition on contact lenses: the past, the present, and the future. Cont Lens Anterior Eye, 2012,35(2): 53-64. DOI: 10.1016/j.clae.2011.12.005.
|
[19] |
Chacon D, Hsieh Y-L, Kurth MJ, et al. Swelling and protein absorption/desorption of thermo-sensitive lactitol-based
|
[21] |
Omali NB, Heynen M, Subbaraman LN, et al. Impact of lens care solutions on protein deposition on soft contact lenses.
|
|
OptomVis Sci, 2016, 93(8): 963-972. DOI: 10.1097/OPX.0000000000000928.
|
|
polyether polyol. Polymer, 2000, 41(23): 8257-8262.
|
[20] |
Luensmann D, Jones L. Protein deposition on contact lenses: the past, the present, and the future. Cont Lens Anterior Eye, 2012,35(2): 53-64. DOI: 10.1016/j.clae.2011.12.005.
|
[22] |
Zhu H, Bandara MB, Vijay AK, et al. Importance of rub and rinse in use of multipurpose contact lens solution. Optom Vis
|
|
Sci, 2011, 88(8): 967-972. DOI: 10.1097/OPX. 0b013e31821bf976.
|
[21] |
Omali NB, Heynen M, Subbaraman LN, et al. Impact of lens care solutions on protein deposition on soft contact lenses.
|
|
OptomVis Sci, 2016, 93(8): 963-972. DOI: 10.1097/OPX.0000000000000928.
|
[22] |
Zhu H, Bandara MB, Vijay AK, et al. Importance of rub and rinse in use of multipurpose contact lens solution. Optom Vis
|
|
Sci, 2011, 88(8): 967-972. DOI: 10.1097/OPX. 0b013e31821bf976.
|
|
|
|