|
|
|
| Density and Morphology of Microglia in Optic Nerve Following Optic Nerve Crush in Mice |
| Yuanfei Ji, Chen Yang, Min Wang, Wencan Wu, Bo Yu |
| Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China |
|
|
|
| Guide |
|
|
Abstract Objective: To investigate the density and morphology of microglia in optic nerve of mice at differenttime points after optic nerve crush. Methods: In this experimental study, thirty-two healthy adult male CX3CR1?/GFP transgenic mice were randomly grouped to normal control group, optic nerve crush (ONC) 1 day group, ONC 7 days group, ONC 14 days group according to the random number table, with 8 mice in each group. ONC models were established in the left eye, while the right eye was not treated, and normal control group without any treatment. Three frozen sections (30 μm) were taken from each optic nerve.Images were taken from each section at 500 μm from optic disk. The density and morphology of microglia in optic nerve were assayed and compared. Data were analyzed by one-way ANOVA. Results: Thenumber of microglia in optic nerve was (438±16) cells/mm2,(323±15) cells /mm2, (1 252±107) cells /mm2, (1 474±113) cells /mm2 in the normal control group, ONC 1 day group, ONC 7 days group, ONC 14 days group. The microglia number significantly increased in ONC 7 days group and ONC 14 days group incomparison with the normal control group and ONC 1 day group (all at P<0.001). In the control group,the nuclei of microglia were small, and the processes were elongated. one day after optic nerve injury,the number of processes decreased, and the change of the nuclear was not obvious. Within 7 days and 14 days after the operation, microglia were largely activated. The number of cells was large, the nucleus was enlarged, and the processing changed from thin to short. Conclusions: Early on, the number of microglia in optic nerve decreased after ONC. Over time, the number of microglia increased greatly, and the morphology changed from ramification to ameoboid.
|
|
Received: 24 December 2021
|
|
|
| Fund:Wenzhou Science and Technology Bureau Program (Y2020362) |
|
Corresponding Authors:
Bo Yu, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China(Email: yubo312@126.com)
|
|
|
|
|
|
|
