基于网络药理学和生物信息学探讨龙胆泻肝汤治疗葡萄膜炎的分子机制

doi:10.3760/cma.j.issn.1674-845X.2018.08.006.

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摘要

目的：探讨龙胆泻肝汤治疗葡萄膜炎的药效学研究和分子机制分析。方法：实验研究。在体实验验证龙胆泻肝汤治疗葡萄膜炎的疗效，利用中药网络药理学数据和分析平台查询并筛选复方龙胆泻肝汤的入血有效成分，并进行网络拓扑分析其可能的治疗靶点。通过查询多种已知疾病靶点数据库，筛选葡萄膜炎的治疗靶点，并与有效成分治疗靶点构建出有效成分-疾病治疗靶点网络，并筛选出关键节点；拓扑分析关键节点参与的信号通路并进行富集分析，筛选出中药复方龙胆泻肝汤治疗葡萄膜炎的可能信号通路。结果：在体实验结果证实龙胆泻肝汤能够减轻大鼠结膜充血、虹膜粘连，减轻前房、睫状体和视网膜炎症细胞浸润，具有治疗葡萄膜炎的的作用；网络药理学分析显示龙胆泻肝汤治疗葡萄膜炎主要通过MAPK信号通路、促性腺激素信号通路和雌激素信号通路，减少炎症因子的产生和细胞浸润，调节机体免疫状况，调控葡萄膜炎的发生发展。结论：龙胆泻肝汤治疗葡萄膜炎可能主要是通过MAPK信号通路、促性腺激素信号通路和雌激素信号通路，调控炎症细胞和炎症因子、自身免疫等来实现。

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关键词 ：龙胆泻肝汤, 葡萄膜炎, 中药复方, 网络药理学

Abstract：

Objective: To study the molecular mechanism of LongdanXiegan Tang for treating uveitis. Methods:This was a experimntal study. The Chinese herbal medicine pharmacology data and analysis platform were used to search and to screen the effective components of a compound injection of LongdanXiegan Tang, and to analyze its possible therapeutic targets based on the network topology. By searching a variety of known disease target databases, we can screen the therapeutic target of uveitis disease and construct the active ingredient-disease treatment target network with the target of effective component treatment and to screen out the key nodes. Using topological analysis of the key nodes involved in the signalpathways and enrichment analysis, and screening out the possible Chinese medicine compound Radix LongdanXiegan Tang for the treatment of uveitis, may help to identify the signal pathways. Results: After the main active ingredient for screening and target prediction of LongdanXiegan Tang were completed, the therapeutic target of uveitis was screened, and an enrichment analysis of the signal pathways was completed. LongdanXiegan Tang can reduce the production of inflammatory cytokines and infiltration and affect the immune status, regulating the occurrence and development of uveitis by mainly regulating the MAPK signaling pathway and promoting both the GnRH and estrogen signaling pathways. Conclusions: LongdanXiegan Tang can treat uveitis primarily through the MAPK, GnRH and estrogen signaling pathways, regulating the inflammatory cells and inflammatory cytokines and autoimmunity.

Key words： LongdanXiegan Tang uveitis traditional Chinese medicine compound network pharmacology

收稿日期: 2018-04-19

基金资助:

国家自然科学基金（81674032, 81373826）；山东省中医药科技发展计划（2015-147）

通讯作者: 毕宏生（ORCID：0000-0002-6965-9626），Email：hongshengbi1@126.com；李丽杰，Email：yankeboshi@163.com

引用本文:

张涛,宋继科,景常莹,蒋文君,吴建峰,解孝锋,李丽杰,毕宏生. 基于网络药理学和生物信息学探讨龙胆泻肝汤治疗葡萄膜炎的分子机制[J]. 中华眼视光学与视觉科学杂志, 2018, 20(8): 484-491. Tao Zhang1, 2, Jike Song1, 2, Changying Jing1, 2, Wenjun Jiang2, 3, Jianfeng Wu1, 2, 3, Xiaofeng Xie2, 4, Lijie Li5,. The Molecular Mechanism of LongdanXiegan Decoction in Treating Uveitis Based on Network Pharmacology and Bioinformatics. Chinese Journal of Optometry Ophthalmology and Visual science, 2018, 20(8): 484-491. DOI: 10.3760/cma.j.issn.1674-845X.2018.08.006.

链接本文:

https://www.cjoovs.com/CN/10.3760/cma.j.issn.1674-845X.2018.08.006. 或 https://www.cjoovs.com/CN/Y2018/V20/I8/484

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