1Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Jinan 250002, China; 2Shandong University of Traditional Chinese Medicine, Jinan 250014, China; 3Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, China; 4Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan 250002, China
Abstract：Objective:To investigate the effects of total glucosides of paeony (TGP) on natural killer T (NKT) cell gene expression in liver of experimental autoimmune uveitis (EAU) rats. Methods:Experimental study. Forty-five Lewis rats were randomly divided into three groups: normal controls, EAU-stimulated rats (model group), and EAU-stimulated rats treated with TGP (TGP intervention group). Control animals received an emulsion of phosphate-buffered saline (PBS) and CFA, which was injected subcutaneously. The other two groups were injected an equal volume of chylomicron containing interphotoreceptor retinoid-binding protein polypeptide (IRBP). Inflammation was observed by daily slit-lamp microscopy of the anterior segments after immunization. The incidence of inflammation and the inflammation scores were recorded. At 12 days after immunization, we extracted the eyes for pathologic examination, and CD4+ T cells were collected from the livers. The expression levels of NKT cells were detected by flow cytometry. Reverse transcription polymerase change reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to measure the gene and protein expression levels of interleukin (IL)-4 and interferon (IFN)-γ. Data were analyzed by one-way ANOVA. Results:Twelve days after immunization, the inflammatory score and pathological grade of the TGP group were significantly lower than in the EAU group, but higher than in the normal group. Flow cytometry showed that compared with normal control group, the number of liver NKT cells in the EAU group and in the TGP intervention group were significantly increased. Further, the increased in the number of NKT expression in the TGP intervention group was higher than in the EAU group (P<0.05) during the inflammatory phase. RT-PCR showed that on day 12 the expression of IFN-γ mRNA in the liver of the TGP intervention group was significantly higher than in the control group (P<0.001), but lower than in the EAU group (P<0.001). At the same time, the expression of liver IL-4 mRNA in the TGP group was lower than that of the normal group (P<0.001), but significantly higher than the EAU group (P<0.001). ELISA showed that the liver IFN-γ levels in the TGP group were significantly lower in the EAU group after 12 days (P=0.01). The concentration of liver IL-4 protein was higher in the TGP group than in the EAU group (P<0.001). Conclusions:TGP can treat uveitis in EAU rats by regulating the expression of liver IL-4 and IFN-γ genes and increasing the number of NKT cells.
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Changying Jing, Jike Song, Beibei Wang, QingMei Tian, Xiaofeng Xie, Tao Zhang, Hongsheng Bi. Effect of Total Glucosides of Paeony on the Expression of Natural Killer T Cells in Livers of Experimental Autoimmune Uveitis Rats. Chinese Journal of Optometry Ophthalmology and Visual science, 2018, 20(11): 669-676.
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