Objective  To report the clinical results of femtosecond laser assisted penetrating keratoplasty （FS-PKP）. Methods  It was a retrospective case series study. Six eyes of 6 patients who treated with FS-PKP were enrolled in this study. The vision of them were from hand moving to 0.05， and opacities were all from epithelium to endothelium. During the operation， the grafts were prepared to different shapes according to the character of the affections： 2 cases with Zig-Zag shape， 1 case with Zig-Square， 1 case with mushroom， and another 2 cases with hat shape. Results  During the follow-up period， the visions of all patients were better than preoperative ones. Three of them were better or equal to 0.5， and one was even to 1.0. The astigmatisms were from 2.25 D to 5.00 D at one year after operation. During the operation of one case with Zig-Zag shape， the meta-central cut were happened. Another patient with hat shape experienced high intraocular pressure early after operation. Conclusion  The FS-PKP appears  to be a good alternative surgical method for patients of corneal diseases because of the great manipuility and excellent postoperative results.