Abstract：Objective: To observe the alternations of choroidal and retinal thickness and blood flow in mice with defocused myopia, and to lay the foundation for the studies of the mechanism of choroidal scleral ischemia and hypoxia in the animal model of lens-induced myopic in mice. Methods: In this experimental study, 22 three-week-old C57BL/6J male mice were randomly divided into 2 groups: the normal control group (group I), and the lens-induced 28 days group (group II), with 11 mice in each group. Group I was the group without any treatment for 28 days. The right eye of group II was defocused by the lens for 28 days to establish a lens-induced myopia model, while the left eye was not treated. Optometry were performed and the axial length (AL) were measured using an ophthalmic optical coherence tomography angiography (OCTA) to detect alternations in the choroid, retinal thickness, and blood fow in different areas and layers. The alternations of diopters, AL, choroidal and retinal blood flow and thickness in different levels and regions between the I and II groups were analyzed by t-test. Results: Before molding, the diopters of group I and group II were +4.80±1.03 D and +5.00±1.05 D (t=0.42, P=0.673), respectively. And AL in each group were 3 245±106 μm and 3 252±116 μm (t=0.14, P=0.894), respectively. After 28 days of molding, the diopters of group I and II were +6.13±1.60 D and -0.14±2.80 D (t=5.47, P<0.001), and AL in each group was 3 329±112 μm and 3 438±35 μm (t=2.91, P=0.009), respectively. The choroidal thickness of group II was thinner than group I, and it was most obvious and statistically signifcant at 1 000 μm on the nasal side (t=3.00, P=0.011). The choroidal blood fow density in group II was smaller than that in group I, and the decrease in blood fow density was most obvious and statistically signifcant in the subnasal region (t=2.67, P=0.018). The thickness of the inner layer of the retina in group II was thinner than that in group I, and the mean values of the supranasal, central, nasal, infratemporal, inferior, and subnasal regions were statistically signifcant (t=2.36-3.92, all P<0.05). The thickness of the outer layer of the retina in group II was thicker than group I, and the mean values of other regions and all regions except for the temporal region were statistically significant (t=2.26-4.02, all P<0.05). The full-thickness of the retina between two groups showed no signifcantly difference. And there was also no signifcant difference in blood fow density of the inner layer of the retina between the group II and group I (all P>0.05). Compared with group I, the blood fow density between the outer layer of the retina in group II was higher only in the nasal region (t=2.70, P=0.015). There was no signifcant difference in retinal full-thickness blood fow density between the group II and group I (P>0.05). Conclusions: In lens-induced myopia mice, the inner layer of the retina is thinned and the outer layer is thickened, and the choroid is thinned while its blood fow is reduced. This study provides a theoretical basis for the study of myopia mice related to the choroid, retinal ischemia, and hypoxia.
傅秋满, 李元君, 卢颖, 等. 离焦性近视小鼠脉络膜及视网膜厚度和血流全域量化分析[J]. 中华眼视光学与视觉科学杂志, 2023, 25(9): 699-706.
Qiuman Fu, Yuanjun Li, Ying Lu, et al. Investigation of Changes in the Thickness and Blood Flow of Choroidal and Retinal in Lens-Induced Myopia in Mice . Chinese Journal of Optometry Ophthalmology and Visual science, 2023, 25(9): 699-706. DOI: 10.3760/cma.j.cn115909-20230307-00058
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