Objective To study the expression of IL-1 beta on the ocular surface of dry eye patients and to study its correlation with symptoms and signs of dry eye； to investigate IL-1 beta′s effect on dry eye pathogenesis. Methods Clinical trials. In this case-control study， 30 dry eye patients （60 eyes） and 15 age- and gender-matched control subjects （30 eyes） without symptoms or signs of dry eye were selected from patients who had been treated in our hospital from September 2012 to February 2013. All subjects underwent full ophthalmologic examinations （including OSDI questionnaire， Schirmer′s test （SIT）， tear break-up time （BUT）， corneal fluorescence staining and conjunctival lissamine green staining）. Impression cytology was used to collect conjunctiva epithelial cells from dry eye patients and controls， then these samples were stained by IHC reaction to detect the expression of IL-1 beta by real-time PCR. Results The average OSDI questionnaire scores for the case group and controls were 24.1±2.2 and 14.3±1.3. SIT scores were 4.13±1.68 mm and 10.53±0.74 mm. BUT times were 4.17±1.10 s and 8.80±1.21 s. Corneal fluorescence staining results were 4.3±1.5 and 0.6±0.5. Conjunctival lissamine green staining results were 5.7±2.0 and 1.9±1.4. There were significant differences between dry eye patients and control subjects （t=22.23， -19.88， -18.20， 12.85， 9.62， P<0.05）. The average expression of IL-1 beta in the case group and controls was 0.65±0.37 and 0.22±0.06. There were significant differences between dry eye patients and control subjects （t=6.31， P<0.05）. The expression of IL-1 beta was positively correlated with scores on the OSDI questionnaire （r=0.81， P<0.05）， corneal fluorescence staining （r=0.58， P<0.05）， and conjunctival lissamine green staining （r=0.48， P<0.05）， and was negatively correlated with BUT （r=-0.45， P<0.05） and SIT （r=-0.43， P<0.05）. With IHC staining， brown particles could be seen in the conjunctiva cells in patients with dry eye， but not in the controls. Conclusion IL-1β as an inflammatory mediator is involved not only in dry eye disease， but is also associated with the severity of dry eye and damage on the ocular surface.
高雅,李冰,陈研遐,常静,郑晓汾. 白介素1β在干眼患者眼表的表达[J]. 中华眼视光学与视觉科学杂志, 2014, 16(4): 228-232.
Gao Ya,Li Bing,Chen Yanxia,Chang Jing,Zheng Xiaofen. A study of the expression of IL-1 beta on the ocular surface in dry eye patients. Chinese Journal of Optometry Ophthalmology and Visual Science, 2014, 16(4): 228-232. DOI: 10.3760/cma.j.issn.1674-845X.2014.04.009
The definition and classification of dry eye disease： report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop （2007）[J]. Ocul Surf，2007，5：75-92.
Jones DT ， Monroy D， Ji Ｚ， et al. Alterations of ocular surface gene expression in Sjogren′s syndrome[J]. Adv Exp Med Biol，1998，438：533-536.
[4]
Pflugeflder SC， Jones D， Ji Z， et al. Altered cytokine balance in zhe tear fluid and conjunetiva of patients with Sjogren′s syndrome keratoeonjunetivitis sicca[J]. Curr Eye Res，1999，19：201-211.
[5]
Inarello CA. Biologic basis for interleukin-1 in desease[J]. Blood，1996，15，2095-2147.
[6]
Solomon A， Dursun D， Liu Z， et al. Pro-and anti-inflammation forms of interleukin-1 in the tear fluid and conjunctiva of patients with dry-eye disease[J]. Invest Ophthalmol Vis Sci，2001，42：2283-2292.
[7]
Niederkorn JY， Stern ME， Pflugfelder SC， et al. Desiccating stress induces T cell-mediated Sjögren′s syndrome-like lacrimal keratoconjunctivitis[J]. J Immunol，2006，176：3950-3957.
[8]
El Annan J， Chauhan SK， Ecoiffier T， et al. Characterization of effector T cells in dry eye disease[J]. Invest Ophthalmol Vis Sci，2009，50：3802-3807.
[9]
De Paiva CS， Chotikavanich S， Pangelinan SB， et al. IL-17 disrupts corneal barrier following desiccating stress[J]. Mucosal Immunol，2009，2：243-253.
[10]
Zheng X， de Paiva CS， Li DQ， et al. Desiccating stress promotion of Th17 differentiation by ocular surface tissues through a dendritic cell mediated pathway[J]. Invest Ophthalmol Vis Sci，2010，51：3083-3091.
[11]
Chauhan SK， El Annan J， Dana R， et al. Autoimmunity in dry eye is due to resistence of Th 17 to Treg suppression[J]. J Immuol，2009，182：1247-1252.
[12]
Dinarello CA. Interleukin-1 beta[J]. Crit Care Med，2005，33：460-462.
[13]
Limb GA， Little BC， Meager A， et al. Cytokines in proliferative vitreoretinopathy[J]. Eye，1991，5：686-693.
Solomon A， Dursun D， Lin Z， et al. Pro- and anti-inflammatory forms of interleukin-1 in the tear fluid and conjuctiva of patients with dry eye disease[J]. Invest Ophthalmol Vis Sci，2001，42：2283-2292.
Gorelik L， Flavell RA. Abrogation of TGF-β signaling in T cells leads to spontaneous T cell differentiation and autoimmune disease[J]. Immunity，2000，12：171-181.
浙公网安备 33030202001892号