Objective Investigate the change of biomechanical properties in porcine sclera treated with genipin and to explore a potential method to prevent the progerssion of myopia. Methods Experimental study. Forty-five porcine cadaver eyes were randomly divided into 9 groups and each group had 5 eyes. Eyes were treated with genipin at concentrations (by w/v) of 0.01%, 0.10%, 1.00% for 30 min. Riboflavin/ultraviolet-A (UVA) treated and untreated samples were used as controls. After treatment, scleral strips of 4.0 mm×10.0 mm were cut. At 24 h after treatment, the stress-strain parameters of the strips were measured using a INSTRON 3343 biomaterial microtester. Additionally, we also performed the stress-strain test at 1, 6, 12, 24 and 36 h after treated with 1.00% genipin. Meanwhile the discoloration of sclera were observed and recorded with slit lamp camera system. The difference of the ultimate stress and modulus between treated and untreated groups were analyzed with One-Way ANOVA in SPSS 17.0. Results Compared with untreated groups, after treatment with genipin for 30 min, the ultimate stress and modulus were increased by 205% to 498% and 109% to 467% respectively. 0.01% genipin for 30min had a similar stress-strain curve with those of eyes treated with riboflavin/UVA. Within 24 h after treating with 1.00% genipin, the sclera exhibited a bluish color which became deeper with time. And at 1, 6, 12, 24 and 36 h after treatment, the ultimate stress were increased by 53%, 146%, 164%, 223% and 175% respectively. The modulus at 8% strain also increased by 80%, 151%, 185%, 352% and 296%. Conclusion Collagen crosslinking treated with genipin could increase the biomechanical strength in porcine sclera. The effect depends on the concentration of genipin.
刘太祥,吴君舒,顾宇伟,杨斌,王铮. 京尼平诱导猪眼巩膜胶原交联后生物力学性能变化[J]. 中华眼视光学与视觉科学杂志, 2014, 16(5): 274-278.
Liu Taixiang,Wu Junshu,Gu Yuwei,Yang Bin,Wang Zheng. Change of biomechanical properties in porcine sclera treated with genipin. Chinese Journal of Optometry Ophthalmology and Visual Science, 2014, 16(5): 274-278. DOI: 10.3760/cma.j.issn.1674-845X.2014.05.004
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