Objective To compare the biomechanical properties of rabbit and human sclera before and after riboflavin/ultraviolet A （UVA） collagen crosslinking. Methods Experimental study. Eight rabbits and 8 human eyeballs were included. The eyes were bisected， one half was cross-linked with riboflavin/UVA （photosensitizer： 0.1% riboflavin drops for 15 minutes， UVA： 3 mW/cm2， 365 nm， and 40 minutes） and the other half served as control. Biomechanical properties testing consisted of ultimate stress， stress and Young′s modulus at 8% strain. Data were analyzed using t test. Results In untreated rabbit and human sclera， Young′s modulus at 8% strain was （4 460±4 090）kPa and （14 310±8 560）kPa， the stress was （359±305）kPa and （1 079±646）kPa， the ultimate stress was （3 830±2 870）kPa and （4 450±1 120）kPa. In rabbit sclera， the stress， ultimate stress and Young′s modulus were significantly increased by riboflavin/UVA crosslinking （t=3.07， 3.50， 3.04， P＜0.05）. The biomechanical properties of human sclera were not statistically affected by crosslinking. Conclusion Human sclera has a higher biomechanical stiffness than rabbit sclera. With the same irradiation dose， riboflavin/UVA crosslinking increases the biomechanical stiffness of rabbit sclera， but not human sclera.
张亚丽,李志伟,牟国营,刘蕾. 核黄素/紫外线A诱导的胶原交联对兔和人巩膜生物力学性能的影响[J]. 中华眼视光学与视觉科学杂志, 2014, 16(5): 279-281.
Zhang Yali,Li Zhiwei,Mu Guoying,Liu Lei. Comparison of biomechanical properties of rabbit and human sclera after riboflavin/ultraviolet A collagen crosslinking. Chinese Journal of Optometry Ophthalmology and Visual Science, 2014, 16(5): 279-281. DOI: 10.3760/cma.j.issn.1674-845X.2014.05.005
Wollensak G， Spoerl E， Seiler T. Riboflavin/ultraviolet-a-induced collagen crosslinking for the treatment of keratoconus[J]. Am J Ophthalmol，2003，135：620-627.
[2]
Vinciguerra P， Albé E， Frueh BE， et al. Two-year corneal cross-linking results in patients younger than 18 years with documented progressive keratoconus[J]. Am J Ophthalmol，2012， 154：520-526.
[3]
Salgado JP， Khoramnia R， Lohmann CP， et al. Corneal collagen crosslinking in post-LASIK keratectasia[J]. Br J Ophthalmol，2011，95：493-497.
[4]
Wollensak G， Spoerl E. Collagen crosslinking of human and porcine sclera[J]. J Cataract Refract Surg，2004，30：689-695.
[5]
Zhang Y， Zou C， Liu L， et al. Effect of irradiation time on riboflavin-ultraviolet-A collagen crosslinking in rabbit sclera[J]. J Cataract Refract Surg，2013，39：1184-1189.
[6]
Wollensak G， Iomdina E， Dittert DD， et al. Cross-linking of scleral collagen in the rabbit using riboflavin and UVA[J]. Acta Ophthalmol Scand，2005，83：477-482.
[7]
Cruysberg LP， Nuijts RM， Geroski DH， et al. The influence of intraocular pressure on the transscleral diffusion of high-molecular-weight compounds[J]. Invest Ophthalmol Vis Sci，2005， 46：3790-3794.
[8]
Ambati J， Canakis CS， Miller JW， et al. Diffusion of high molecular weight compounds through sclera[J]. Invest Ophthalmol Vis Sci，2000，41：1181-1185.
[9]
Nicoli S， Ferrari G， Quarta M， et al. Porcine sclera as a model of human sclera for in vitro transport experiments： histology， SEM， and comparative permeability[J]. Mol Vis，2009，15：259-266.
[10]
Olsen TW， Edelhauser HF， Lim JI， et al. Human scleral permeability. Effects of age， cryotherapy， transscleral diode laser， and surgical thinning[J]. Invest Ophthalmol Vis Sci，1995， 36：1893-1903.
[11]
Wollensak G， Iomdina E. Long-term biomechanical properties of rabbit sclera after collagen crosslinking using riboflavin and ultraviolet A （UVA）[J]. Acta Ophthalmol，2009，87：193-198.
[12]
Wollensak G， Spoerl E， Seiler T. Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking[J]. J Cataract Refract Surg，2003，29：1780-1785.
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