Objective To assess the accuracy of axial length (AL), corneal curvature (K1, K2 and Km) and anterior chamber depth (ACD) measurements with the Biograph/Lenstar LS 900 biometer and the IOL Master. Methods In this prospective clinical study, 68 eyes of 34 myopes, whose spherical equivalent were -0.50-10.00 D, with an average of (-4.11±2.31)D. AL, K1, K2, Km and ACD of these myopes were measured with the Biograph/Lenstar and IOL Master biometer. Data were analyzed using a paired samples t test, linear regression and Bland-Altman plots. Results The mean AL and ACD measured by the Biograph/Lenstar and IOL Master were (25.39±1.09)mm, (25.37±1.08)mm, and (3.80±0.26)mm, (3.78±0.26)mm, respectively. There were significant differences between the results of these two methods in AL (t=8.855, P＜0.01), K1, K2 and Km (t=-2.511, P=0.014;t=-2.413, P=0.019; t=-2.893, P=0.005), but not for ACD (t=1.073, P=0.287). A Pearson correlation revealed a high correlation for AL, corneal curvature and ACD measurements between the two devices tested (r＞0.9 for all, P＜0.01 for all). The Bland-Ahman showed that the two devices had non-comparable results for corneal curvature but comparable results for AL and ACD. Conclusion The AL and ACD values obtained with the Biograph/Lenstar are correlated very well to those of the IOL Master. It is important to note in clinical practice, that corneal curvature values acquired by these devices are not directly interchangeable.